The paper deals with the primary objective to optimize different parameters such as concentration, temperature, pH, time and size of cells for the decolorization of azo dye (Congo red) by using bacterial isolates by the method of immobilized bacterial cells. Staphylococcus aureus immobilized beads show a maximum dye decolorization of 94% under optimum condition and found to be more efficient in dye decolorization. The optimum value for degradation is found to be 35°C, for 400 mg/L of concentration of dye at the medium pH of 7. The order of the reaction was found to be first order with the rate constant of 0.073 h^-1. The dye adsorbed on the bead surface follows Freundlich adsorption isotherm with the constant values are K = 1.368 and 1/n = 0.773. Studies have been carried out by FTIR, GC-MS analysis for confirming the biodegradation of Congo Red by the immobilized bacterial cells. The results reported warrant further investigation to establish the usefulness of these isolates for bioremediation and biodegradation application such as wastewater treatment.

中文翻译：

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固定化藻酸盐珠上细菌细胞培养的稳定和刚果红脱色的优化

本文的主要目的是通过固定化细菌细胞的方法，通过使用细菌分离物来优化不同的参数，例如浓度，温度，PH，时间和细胞大小，以使偶氮染料（刚果红）脱色。在最佳条件下，固定有金黄色葡萄球菌的珠子显示最大的染料脱色率为94％，并且发现在染料脱色方面更有效。发现在介质p H为7时，对于400 mg / L的染料浓度，降解的最佳值为35°C。发现反应的顺序为一级，速率常数为0.073 h ^-1。吸附在珠粒表面的染料遵循Freundlich吸附等温线，常数为K = 1.368和1 / n = 0.773。已经通过FTIR，GC-MS分析进行了研究，以证实固定化细菌细胞对刚果红的生物降解作用。报告的结果值得进一步研究，以建立这些分离物在生物修复和生物降解应用（如废水处理）中的有用性。