Transcription factors (TFs) are protein, which perform their role at transcriptional level by affecting the expression of various genes associated with metabolic pathways, growth and stress-associated genes (SAGs) at different developmental stages. Here, we identified 38 novel heat-responsive transcription factor genes from wheat cv. HD2985 by mining the de novo transcriptome data derived from heat shock (HS) treated wheat. Based on digital gene expression (DGE), a putative transcript (TaHD97) of ~1.1 kbas amplified and cloned from wheat cv. HD2985. The presence of heat stress transcription factor (HSF) DNA binding domain was observed in the amino acid sequence. Differential expression of TaHD97 was observed in HD2985 (thermotolerant) and HD2329 (thermosensitive) under heat stress. Tissue specific expression analysis showed up-regulation of TaHD97 in leaves, stem and endospermic tissues and down-regulation in root under HS. A positive correlation was established between the expression of TaHD97 and its target gene (HSP17 and HSP90) in wheat under heat stress. HSP17 transcripts were observed more in leaves of HD2985, as compared to HD2329. Thermotolerance related biochemical enzymes (SOD, CAT, GPX and TBARS) were observed higher in wheat cv. HD2985 showing maximum expression of TaHD97 under heat stress. There is a need for the functional validation of the gene TaHD97 in order to use it for the regulation of sHSP (catalytic chaperone) - a novel approach towards augmenting thermotolerance in wheat under heat stress.

中文翻译：

---

提供耐热性的小麦（Triticum aestivum）的推定热响应转录因子（TaHD97）及其靶标

转录因子（TFs）是蛋白质，它通过影响与不同发育阶段的代谢途径，生长和应激相关基因（SAG）相关的各种基因的表达，在转录水平上发挥其作用。在这里，我们确定了小麦cv的38个新的热响应转录因子基因。HD2985通过挖掘德从头从热休克（HS）处理的小麦衍生转录数据。基于数字基因表达（DGE），从小麦cv中扩增并克隆了约1.1 kbas的推定转录本（TaHD97）。HD2985。在氨基酸序列中观察到热应激转录因子（HSF）DNA结合域的存在。TaHD97的差异表达在热应力下，在HD2985（耐热）和HD2329（热敏）中观察到。组织特异性表达分析表明，在HS下，叶片，茎和胚乳组织中TaHD97的表达上调，而根中的表达下调。在热胁迫下，小麦中TaHD97的表达与其靶基因HSP17和HSP90的表达呈正相关。与HD2329相比，在HD2985的叶子中观察到更多的HSP17转录本。小麦cv中耐热性相关的生化酶（SOD，CAT，GPX和TBARS）较高。HD2985表示的最大表达TaHD97热胁迫下。需要基因的功能验证为了将其用于sHSP（催化伴侣）的调节，需要使用TaHD97-一种提高热胁迫下小麦耐热性的新方法。