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Estimation of genetic diversity, structure and trait association of winged bean (Psophocarpus tetragonolobus (L.) DC.), genotypes through AFLP and ITS markers
Indian Journal of Biochemistry and Biophysics ( IF 1.5 ) Pub Date : 2020-01-03
Chandra Sekhar Mohanty, Vinayak Singh, Priya Gupta, Anukool Srivsatava, Rojalin Pattanayak, Vandana Jaiswal, Nilamani Dikshit

The genetic diversity among ninety five accessions of winged bean (Psophocarpus tetragonolobus (L.) DC.) belonging to six countries of African and Asiatic origin was detected by employing amplified fragment length polymorphism (AFLP) and internal transcribed spacer (ITS) of nuclear ribosomal DNA markers. A medium to low-level of genetic diversity exists among the accessions of P. tetragonolobus. Association between the AFLP markers and flower, pod and seed traits: days to 50% flowering (DFW), pod length (PDL), pod width (PDW), green pod length (GPL), number of pods per plant (PDSP), number of seeds per pod (SDPD), 100 seed weight (SWT) and seed-oil content (SOC) was carried out by employing the mixed linear model (MLM) based kinship matrix (K-model). Traits like SOC and PDW were found to be strongly associated with AFLP markers. Population structure analysis among the genotypes identified five discrete sub-populations among the studied African and Asiatic lines of P. tetragonolobus.

中文翻译:

通过AFLP和ITS标记估算四棱豆(Psophocarpus tetragonolobus(L.)DC。)的遗传多样性,结构和性状关联,基因型

通过利用核糖体的扩增片段长度多态性(AFLP)和内部转录间隔区(ITS)检测了属于非洲和亚洲六个国家的九十五个四棱豆Psophocarpus tetragonolobus(L.)DC。)的遗传多样性。 DNA标记。四角龙虾的种质中存在中等至低水平的遗传多样性。AFLP标记与花,荚果和种子性状之间的关联:开花至50%的天数(DFW),荚果长度(PDL),荚果宽度(PDW),绿色荚果长度(GPL),单株荚果数(PDSP),通过使用基于混合线性模型(MLM)的亲属矩阵(K-model)进行每荚种子数(SDPD),100种子重量(SWT)和种子油含量(SOC)的计算。发现SOC和PDW等性状与AFLP标记密切相关。基因型之间人口结构分析鉴定的研究非洲和亚洲线中五个独立亚群P. tetragonolobus
更新日期:2020-01-03
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