The objective of the present research is to envisage microbial assorted variety in the reactor through systems of general polymerase chain reaction (PCR) and real-time PCR, which can be further applied for production of methane using anaerobic reactor by employing vegetable waste as a feed. The outcomes proposed the names of species identified with the hydrolytic microorganisms, for example, Bacillus subtilis and hydrogenotrophic methanogenic archaea like i.e. Methano culleus and i.e. Methano corpusculum of the methanogenic microbes. Results demonstrated that, presence of methanogenic archaea in the reactor was confirmed using general PCR.

中文翻译：

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聚合酶链反应和实时PCR参数用于扩增厌氧生物反应器中的水解微生物和氢营养型产甲烷菌

本研究的目的是通过通用聚合酶链反应（PCR）和实时PCR系统设想反应器中的微生物种类，该方法可进一步利用植物性废料作为厌氧反应器来生产甲烷。 。结果提出了水解微生物鉴定物种的名称，例如，枯草芽孢杆菌和氢营养产甲烷古细菌等即产甲烷culleus和即产甲烷corpusculum产甲烷微生物。结果表明，使用常规PCR证实了反应器中产甲烷菌的存在。