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Genetic polymorphism in Dalbergia sissoo Roxb. using RAPD markers
Indian Journal of Biochemistry and Biophysics ( IF 1.476 ) Pub Date : 2019-08-08
Shivani Dobhal, Shruti Sharma, Nafeesh Ahmed, Ashok Kumar

Genetic diversity in forty nine genotypes of Dalbergia sissoo Roxb. was analyzed using randomly amplified polymorphic DNA (RAPD) with 10 decamer primers. A total of 129 distinct DNA fragments (bands) were amplified, of which 109 bands were polymorphic ranged from 200 to 1300 base pair. In fact, the number of amplified bands per genotypes varied from 7 to 14 and polymorphism percentage ranged from 63.63 to 93.33 with an average of 84.93. The mean polymorphic information content (PIC) for RAPD primers was recorded to be 0.24 which ranged from 0.24 to 0.33. The Jaccard similarity coefficient ranged from 0.62 to 0.93 showing wide range of variability among the genotypes. The unweighted pair group method with arithmetic averages (UPGMA) and principle coordinate analysis (PCoA) depicted relationship among the genotypes, which were found to be complimentary to each other. The maximum genetic diversity (0.38) was reported between genotypes FRI/DS/7006 and FRI/DS/0059, whereas genotypes FRI/DS/0218 and FRI/DS/1003 were found to be least divergent (0.07). The genotypes were grouped into eight clusters, off which, cluster II contained maximum twenty genotypes, and cluster VII and VIII comprised just one genotypes each.

中文翻译:

Dalbergia sissoo Roxb的遗传多态性。使用RAPD标记

黄檀的49种基因型的遗传多样性Roxb。使用带有10个decamer引物的随机扩增多态性DNA(RAPD)进行分析。总共扩增了129个不同的DNA片段(条带),其中109个条带的多态性介于200至1300个碱基对之间。实际上,每个基因型的扩增条带数量在7到14之间变化,多态性百分比在63.63到93.33之间,平均为84.93。RAPD引物的平均多态信息含量(PIC)被记录为0.24,范围从0.24到0.33。Jaccard相似系数介于0.62至0.93之间,显示了基因型之间的广泛差异。具有算术平均值(UPGMA)和主坐标分析(PCoA)的非加权对分组方法描述了基因型之间的关系,发现它们之间是互补的。最大遗传多样性(0。在基因型FRI / DS / 7006和FRI / DS / 0059之间报告了38),而基因型FRI / DS / 0218和FRI / DS / 1003之间的差异最小(0.07)。基因型分为八类,其中第二类最多包含二十种基因型,第七类和第八类仅包含一种基因型。
更新日期:2019-08-08
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