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Microsatellite (SSR) markers assisted characterization of rice (Oryza sativa L.) genotypes in relation to salt tolerance
Indian Journal of Biochemistry and Biophysics ( IF 1.476 ) Pub Date : 2019-08-08
Bibha Rani, V K Sharma

Under present investigation a total of 18 rice genotypes with diverse genetic background were taken for screening at seedling stage and its molecular characterization using SSR markers. Selected rice genotypes were exposed to five salinity levels (0, 2, 4, 6 and 8 dSm-1) at seedling stage. Modified standard evaluation score (SES) at seedling stage identified 7 highly tolerant, 2 tolerant and 8 moderately tolerant genotypes. The susceptible genotype was IR64 with score 7. Considering 4 other morpho-physiological characters (germination percent, K/Na ratio, shoot and root length, shoot and root fresh weight, shoot and root dry weight) studied at seedling stage, the genotype CSR2K-262 showed greater tolerance among all the 18 genotypes. For molecular characterization a total of 44 SSR markers were selected. The average number of alleles per locus was 9.3 indicating greater magnitude of diversity among plant materials. The average PIC value 0.767 confirmed that the markers used were highly informative. The cluster analysis grouped the 18 genotypes into three major clusters. Cluster I was the largest with 9 genotypes with all highly tolerant and tolerant genotypes, Cluster II comprised 8 genotypes with all tolerant and moderately tolerant and Cluster III comprised of 1 genotype (IR64) identified as susceptible one.

中文翻译:

微卫星(SSR)标记有助于鉴定水稻(Oryza sativa L.)基因型与耐盐性的关系

在目前的调查中,共有18种具有不同遗传背景的水稻基因型在苗期进行了筛选,并使用SSR标记对其进行了分子鉴定。选定的水稻基因型暴露于五个盐度水平(0、2、4、6和8 dSm -1)在苗期。苗期改良的标准评价分数(SES)鉴定了7种高度耐受,2种耐受和8种中等耐受的基因型。易感基因型为IR64,得分为7。考虑到苗期研究的其他4种形态生理特征(发芽率,K / Na比,枝和根长,枝和根鲜重,枝和根干重),基因型CSR2K -262在所有18个基因型中表现出更大的耐受性。为了进行分子表征,总共选择了44个SSR标记。每个基因座的平均等位基因数量为9.3,表明植物材料之间的多样性更大。PIC平均值0.767证实使用的标记物具有很高的参考价值。聚类分析将18个基因型分为三个主要聚类。
更新日期:2019-08-08
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