Magnetic nanoparticles have been researched extensively recently for site specific cancer cell treatment. Typically, ultrafine, stable and biocompatible particles prepared using simple, economical and environment friendly approaches are greatly desirable. In the present study hematite nanoparticles were prepared by a semi-synthetic approach using aqueous garlic extract. The Allium sativum (as) prepared particles were appropriately characterized and checked for in vitro cytotoxic effects and hemocompatability. Well dispersed (polydispersity index 0.144), superparamagnetic (Ms: 15.677 emu/g), spherical particles possessing an average 10 nm diameter and good colloidal stability (zeta potential: -36.8 mV) were obtained. Nanoparticles displayed a UV absorption maximum at 450 nm and a characteristic peak at 587 cm^-1 in the Infra-red (IR) spectra representing Fe-O bond. Various bioactive components present in the garlic extract could reduce the metal precursors as well as help in coating the nanoparticles. Nanoparticles exhibited negligible percentage hemolysis and concentration dependent cytotoxicity with inhibition concentration fifty percent (IC₅₀) values of 230 µg/ml, 346 µg/ml and 285 µg/ml for cell lines of colorectal cancer (HCT 116), breast cancer (MCF-7) and cervical cancer (HeLa), respectively. In contrast normal lung fibroblast MRC-5 cell line did not exhibit any apparent cytotoxicity in the studied concentration range. Hence, biocompatible magnetic nanoparticles were green synthesized for potential therapeutic application.

中文翻译：

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基于大蒜提取物的尺寸控制超顺磁性赤铁矿纳米颗粒的制备及其细胞毒性应用

磁性纳米粒子最近已被广泛研究用于特定部位的癌细胞治疗。通常，非常需要使用简单，经济和环境友好的方法制备的超细，稳定和生物相容的颗粒。在本研究中，使用含水大蒜提取物通过半合成方法制备了赤铁矿纳米颗粒。的大蒜（如）中制备的颗粒，其特征在于适当地并检查体外细胞毒性作用和血液相容性。获得了良好分散的（多分散指数0.144），超顺磁性（Ms：15.677 emu / g），具有平均10nm直径和良好的胶体稳定性（ζ电势：-36.8mV）的球形颗粒。纳米颗粒在代表Fe-O键的红外（IR）光谱中显示了在450 nm处的最大UV吸收和在587 cm ^-1处的特征峰。大蒜提取物中存在的各种生物活性成分可以还原金属前体，并有助于包覆纳米颗粒。纳米颗粒的溶血百分率和浓度依赖性的细胞毒性微不足道，抑制浓度为百分之五十（IC ₅₀）对于大肠癌（HCT 116），乳腺癌（MCF-7）和子宫颈癌（HeLa）的细胞系，分别为230 µg / ml，346 µg / ml和285 µg / ml。相反，正常的肺成纤维细胞MRC-5细胞系在所研究的浓度范围内没有表现出任何明显的细胞毒性。因此，生物相容性磁性纳米颗粒被绿色合成以用于潜在的治疗应用。