An external loop airlift photobioreactor of 5 litre capacity was fabricated with glass as per the design. Blue green algae Anabaena ambigua was cultured in this external loop airlift photobioreactor using cross-shaped and circular sparger. For each type of sparger different sets of air velocities viz., 1 m/s, 1.5 m/s, 2 m/s, 2.5 m/s and 3 m/s were maintained and, once the culture reached stationary phase, the biomass was estimated by gravimetric method. For cross-shaped sparger maximum biomass was obtained at air velocity of 1.5 m/s and for circular sparger maximum biomass was obtained at air velocity of 2 m/s. Statistical analysis two-way analysis of variance (ANOVA) was performed using Microsoft Excel 2007. It was observed that there is a significant change in the biomass formed by changing the air velocity as well as changing the type of sparger. A maximum biomass of 0.809 g/L was obtained using cross-shaped sparger at air velocity of 1.5 m/s.

中文翻译：

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十字形和圆形分布器在外环气举光生物反应器中生长的鱼腥藻生物量的比较研究

根据设计，用玻璃制造了容量为5升的外环气举光生物反应器。蓝绿藻Anabaena ambigua使用十字形和圆形喷头在此外环式气举光生物反应器中进行培养。对于每种类型的喷头，保持不同的风速，分别为1 m / s，1.5 m / s，2 m / s，2.5 m / s和3 m / s，一旦培养物达到固定相，生物量用重量法估算。对于十字形喷头，在1.5 m / s的风速下获得最大生物量，对于圆形喷头，在2 m / s的风速下获得最大生物量。使用Microsoft Excel 2007进行了统计分析的方差双向分析（ANOVA）。观察到，通过改变风速和改变喷头的类型，形成的生物量发生了显着变化。使用十字形喷头以1.5 m / s的风速获得最大生物量0.809 g / L。