In this study we describe the effects of Pleurotus ostreatus transformants that have been silenced for laccase genes. Using the RNAi strategy, transformants with different levels of phenotypic alteration were obtained with respect to their oxidation capacity of 2,6-dimethoxyphenol (DMP). The analysis of laccase activity on DMP allowed us to select transformants with severe, medium and light phenotypic alteration in comparison to the PoB strain. The measurements of the average growth rate of the transformants with severe phenotypic alteration suggested that laccase expression could play a role in the vegetative growth of P. ostreatus. It was observed that both in solid and liquid cultures, PoB and the transformants express mRNA for lacc10, although the transformants with medium and severe phenotypic alteration present a decrease in intensity, especially in solid culture. This suggests that the product of this gene is responsible for the development of the mycelium and probably participates in the production of biomass in solid culture and also could be related to the decrease in the intensity of the constant isoenzyme observed in both culture systems.

中文翻译：

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漆酶基因沉默负面影响平菇的生长发育。

在这项研究中，我们描述了已被漆酶基因沉默的平菇平菇转化子的作用。使用RNAi策略，就其2，6-二甲氧基苯酚（DMP）的氧化能力而言，获得了具有不同表型改变水平的转化体。对DMP漆酶活性的分析使我们能够选择与PoB菌株相比具有严重，中等和轻型表型改变的转化子。对具有严重表型改变的转化子的平均生长速度的测量表明，漆酶表达可能在平菇的营养生长中起作用。观察到在固相和液体培养中，PoB和转化子均表达lacc10的mRNA，尽管具有中等和严重表型改变的转化子强度降低，特别是在固体培养物中。这表明该基因的产物负责菌丝体的发育，并且可能参与固体培养物中生物量的产生，并且还可能与在两种培养系统中观察到的恒定同工酶强度的降低有关。