Tularemia, commonly prevalent in various regions of the northern side of the equator, is a zoonotic illness caused by Francisella tularensis. Among various Francisella tularensis species, subspecies tularensis is most pathogenic to human and included as category a biowarfare agent which can cause infection through aerosol, cut, injuries in skin and contact with tainted creatures. Right now, there is no approved vaccine is available for this very infectious intracellular pathogen. In this study, three Francisella proteins viz. FopA, GroEL and DnaK were cloned and expressed. The recombinant proteins were purified and utilized as a cocktail for immunization in mice. The ELISA results demonstrated that this cocktail elicited high antibody titres in sera of vaccinated animals. Among the IgG subtypes, IgG1 response was prevalent in immunized animals took after by IgG2. This indicated the induction of Th2 type immune responses against this cocktail a vaccine candidate. The investigation demonstrated that the blend of these three proteins has the potential as vaccine candidate for tularemia.

中文翻译：

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重组FopA，DnaK和GroEL混合液在小鼠模型中作为抗弗氏杆菌的疫苗的体液免疫应答研究

Tularemia通常在赤道北侧的各个地区盛行，是一种由弗朗西斯 菌弗拉索菌引起的人畜共患病。在各种弗朗西斯 土拉弗朗西斯菌的物种，亚种土拉弗朗西斯菌是最致病人类和类包括了生物战剂可通过气溶胶，切，在皮肤接触被感染动物受伤引起感染。目前，尚没有针对这种极具传染性的细胞内病原体的批准疫苗。在这项研究中，三种弗朗西斯菌蛋白即。克隆并表达了FopA，GroEL和DnaK。纯化重组蛋白，并用作鸡尾酒免疫小鼠。ELISA结果表明，该混合物在接种动物的血清中引起高抗体滴度。在IgG亚型中，IgG1反应在被IgG2感染的免疫动物中普遍存在。这表明诱导了针对该混合物的候选疫苗的Th2型免疫反应。研究表明，这三种蛋白质的混合物有可能成为Tularemia的候选疫苗。