Ehrlichiosis and babesiosis are the most pathogenic tick-borne diseases of dogs worldwide. The present study reports that the development of SYBR green based real time PCR (RT-PCR) protocols with novel primers targeting small subunit ribosomal RNA genes to detect natural infections of Ehrlichia canis, Babesia vogeli and B. gibsoni in dogs and its comparison with conventional PCR. Statistical analysis revealed that RT- PCR is more superior to conventional PCR assay to detect low level rickettsaemia (p < 0.05). The high prevalence of these pathogens in the study population also warrants immediate attention to the adoption of efficient and sustainable control strategies.

中文翻译：

---

常规和实时荧光定量PCR检测狗血中寄生虫的比较分析

埃希氏病和巴贝虫病是全世界犬中最具致病性的tick传播疾病。本研究报告开发了基于SYBR green的实时PCR（RT-PCR）方案，该方案具有针对小型亚基核糖体RNA基因的新型引物，可检测犬埃里希氏犬，贝贝小球菌和g.bibsoni的自然感染，并将其与常规方法进行比较PCR。统计分析表明，RT-PCR在检测低水平立克次体血症方面优于常规PCR分析（p <0.05）。这些病原体在研究人群中的高流行也值得我们立即注意采用有效和可持续的控制策略。