Armyworm Mythimna spp. is one of the serious pests of crops belonging to Gramineae family viz. sorghum, millets, rice, maize and wheat in different regions of world. Although different species of Mythimna were existing in different parts of the world, but in Punjab, all populations infesting different host plants are considered as Mythimna separata. Eight samples from different regions of Punjab viz. six infesting rice from Patiala (2), Muktsar¹, Gurdaspur (2) and Bathinda¹; one each from maize and sorghum from Ludhiana and TarnTaran, respectively were analysed. The DNA barcode region (mitochondrial cytochrome oxidase 1) of each sample was cloned and sequenced. Basic Local Alignment Search Tool (BLAST) analysis showed that four samples collected from rice (Patiala-2, Muktsar, Gurdaspur) showed 99% homology with M. loreyi. Two samples from rice (Gurdaspur and Bathinda) and one each from sorghum and maize showed a homology of 99-100% with M. separata. Our results report the occurrence of M. loreyi species in rice ecosystem in Punjab along with M. separata. The phylogenetic analysis of population of this study and population from different countries formed two distinct groups of M. loreyi and M. separata. The maximum genetic distance was 0.008 and 0.004 among the different population of M. loreyi and M. separata, respectively.

中文翻译：

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DNA条码显示印度旁遮普邦Mythimna loreyi（Duponchel）的发生

粘虫粘虫属。是属于禾本科家族的庄稼的严重害虫之一。世界不同地区的高粱，小米，水稻，玉米和小麦。尽管世界各地存在不同种类的Mythimna，但在旁遮普邦，所有侵染不同寄主植物的种群都被认为是Mythimna separata。来自旁遮普邦不同地区的八个样本。来自Patiala（2），Muktsar ¹，Gurdaspur（2）和Bathinda ^1的六种水稻; 分析了Ludhiana和TarnTaran分别来自玉米和高粱的一种。克隆每个样品的DNA条形码区域（线粒体细胞色素氧化酶1）并测序。基本的局部比对搜索工具（BLAST）分析表明，从水稻中收集的四个样品（Patiala-2，Muktsar，Gurdaspur）与罗勒氏酵母具有99％的同源性。水稻中的两个样品（Gurdaspur和Bathinda）以及高粱和玉米中的每个样品均显示与分离支原体的同源性为99-100％。我们的结果报告了旁遮普邦的水稻生态系统中出现了罗伊分枝杆菌以及分离的分枝杆菌。这项研究的人口和来自不同国家的人口的系统发育分析构成了罗勒伊氏菌的两个不同的群体和M. separata。最大遗传距离为0.008和不同人群中0.004 M. loreyi和粘虫，分别。