The aim of present study was to explore microbiology of manganese mining area of Balaghat and Chhindwara, Madhya Pradesh, India with the objective of reducing load of mine based pollution to support environmental sustainability with help of bacterial isolates. The research involves physicochemical analysis, culture dependent methods, 16S rDNA based sequencing and computational phylogenetic analysis. The 16S rDNA sequence analysis revealed the occurrence of two iron oxidizing bacteria (Staphylococcus hominis and Pseudomonas sp.) and four sulphur oxidizing bacteria (Bacillus cereus HYM74, B. anthracis, B. cereus D42 and Pantoea calida) in the selected sites. All cultures were able to grow on acidic as well as neutral pH medium and at low temperature of 4^oC. Bacterial isolates were also found with heavy metal tolerance for Mn⁺⁷ and Cr⁺⁶ up to the concentration of 1000 ppm. This study assists the idea of biomineralization, bioremediation and future reclamation in the selected mining area with the help of bacteria.

中文翻译：

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印度中央邦巴拉格特和钦德瓦拉锰矿区硫和铁氧化细菌的遗传特征

本研究的目的是探索印度中央邦巴拉格特和钦德瓦拉锰矿区的微生物，目的是减少基于矿井的污染负荷，并通过细菌分离物来支持环境可持续性。该研究涉及理化分析，培养相关方法，基于16S rDNA的测序和计算机系统发育分析。16S rDNA序列分析表明，发生了两种铁氧化细菌（人葡萄球菌和假单胞菌）和四种硫氧化细菌（蜡状芽孢杆菌HYM74，炭疽芽孢杆菌，蜡状芽孢杆菌D42和泛菌））。所有培养物都能够在酸性和中性pH培养基上以及在4 ^oC的低温下生长。还发现细菌分离株对Mn ⁺⁷和Cr ^+6的重金属耐受性高达1000 ppm。这项研究有助于在细菌的帮助下在选定矿区进行生物矿化，生物修复和未来的填海。