The cultivation of pepper (Capsicum annuum) is one of the oldest in America. However, out of the 25 species described only 5 have been domesticated. The varieties registered and legally protected must comply with International Union for the Protection of New Varieties of Plants (UPOV) guidelines. Nevertheless, simple sequence repeat the morphological descriptors used for this purpose are sometimes subjective and they are altered by the environment conditions. Molecular genetic markers have been used as alternative to reduce these limitations. The aim of this study was to compare the effectiveness of three techniques based on PCR to discriminate varieties of jalapeño pepper with little phenotypic differences and with very similar shape of the fruit. The techniques used were random amplified polymorphic DNA-DNA amplification fingerprinting (RAPD-DAF), inter simple sequence repeat (ISSR) and simple sequen repeat (SSR) through capillary and conventional electrophoresis. Results indicated that the values of genetic variation in the studied jalapeno varieties are different depending on the technique used, which is due to the capacity of each technique to sample the genome.

中文翻译：

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三种DNA-PCR技术用于区分墨西哥胡椒胡椒品种的有用性

辣椒（辣椒）的栽培）是美国最古老的城市之一。但是，在描述的25个物种中，只有5个已被驯化。已注册并受到法律保护的品种必须符合国际植物新品种保护联盟（UPOV）指南。然而，简单的序列重复用于该目的的形态学描述有时是主观的，并且会因环境条件而改变。分子遗传标记已被用作减少这些限制的替代方法。这项研究的目的是比较三种基于PCR的技术来区分不同表型差异和水果形状非常相似的墨西哥胡椒品种。使用的技术是随机扩增多态性DNA-DNA扩增指纹图谱（RAPD-DAF），通过毛细管电泳和常规电泳进行简单序列重复（ISSR）和简单序列重复（SSR）。结果表明，所研究的墨西哥胡椒品种的遗传变异值因所使用的技术而异，这是由于每种技术对基因组进行采样的能力所致。