Pinus roxburghii Sarg. commonly known as Chir pine, is a commercially exploited species for resin in India. Resin is a phenotypic trait which is expressed only in mature trees. Due to this a large number of trees are cut every year which pose a serious ecological threat. Recent advancements in bioinformatics and molecular biology have enabled scientists to identify high resin yielding pine genotypes at nursery stage using molecular markers. In the present study, investigation, 337 terpene coding sequences from the database were analyzed using data mining and 45 microsatellites allele specific primers were designed for marker assisted selection. Out of these 45 primers, only 22.2% primer pairs showed polymorphism within the 53 genotypes of Chir pine examined. A wide range of fragment size was observed from 100 - 600 bp. PCR amplification using allele specific markers produced a total of 22 bands, out of which 14 were found to be polymorphic. The total number of bands amplified per marker varied from of 1 to 3 with an average of 1.4. Genetic divergence in terms of percent polymorphism ranged from 50 to 100% with a mean of 63.3% per marker.

中文翻译：

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通过数据挖掘鉴定赤松（Pinus roxburghii Sarg。）等位基因特异性引物

樟子松。俗称Chir pine是印度商业上开发的树脂树种。树脂是一种表型性状，仅在成熟树木中表达。因此，每年砍伐大量树木，构成了严重的生态威胁。生物信息学和分子生物学的最新进展使科学家能够在育苗期使用分子标记物鉴定出高产树脂的松树基因型。在本研究中，调查使用数据挖掘技术分析了数据库中的337种萜烯编码序列，并设计了45种微卫星等位基因特异性引物用于标记辅助选择。在这45种引物中，只有22.2％的引物对在所研究的Chir pine的53个基因型中显示出多态性。在100-600 bp范围内观察到很大的片段大小。使用等位基因特异性标记的PCR扩增共产生22条带，其中14条被发现是多态性的。每个标记扩增的条带总数为1至3，平均为1.4。就多态性百分比而言，遗传差异范围为50％至100％，每个标记平均为63.3％。