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Effects of administration of 10 nm or 50 nm gold nanoparticles (AuNPs) on blood profile, liver and kidney functions in male albino rats
Indian Journal of Biochemistry and Biophysics ( IF 1.5 ) Pub Date : 2020-07-27
Sahar H Orabi, Doaa A Mansour, Said I Fathalla, Shaaban M Gadallah, Amina A Gamal ElDin, Ahmed Sabry S Abdoon

This work aimed to investigate the effect of acute and chronic administration of gold nanoparticles (GNPs) on liver and kidney functions, blood glucose concentration, lipid profile, and haematological parameters in male albino rats. Two experiments were conducted. In acute study: Fifty-four adult mature male rats were randomly assigned into three equal groups (18 per group). Group 1 (control group): in which rats were received intramuscular (i.m) injection of 1 ml normal saline 0.9%. Group 2 (50 nm GNPs group): rats were i.m. injected with a single dose of 75 µg 50 nm GNPs/kg body weight (bwt). In Group 3 (10 nm GNPs group): rats were i.m. injected with a single dose of 75 µg 10 nm GNPs/kg bwt. In chronic study: Eighteen adult male rats were randomly divided into three equal groups (6 per group). Group І (control): rats were intramuscular (i.m) repeatedly injected with 1 ml normal saline 0.9% once/week 5 for weeks. Group 2 (50 nm GNPs): rats were i.m. injected with once/week with a dose of 75 µg 50 nm GNPs/kg bwt) for 5 weeks. In Group 3 (10 nm GNPs): male rats were i.m. injected with once/week with a dose of 75 µg 50 nm GNPs/kg bwt for 5 weeks, followed by 3 weeks washout period for all groups. Blood was collected at 3, 7, and 60 days in acute experiment, while, they were collected only before and after 2 months in chronic experiment. Acute and chronic administration of GNPs (10 or 50 nm size) in male albino rats induced no significant alterations for liver and kidney functions, lipid profile parameters and different haematological parameters at days 3 and 60 of the study. However, on day-7 post-treatment, GNPs-treated rats showed significantly (P <0.05) higher serum ALT, AST, ALP, urea, creatinine, glucose, and different lipid profile and decreased HDL level. Chronic administration of 10 nm or 50 nm GNPs significantly (P <0.05) decreased serum glucose levels. In conclusion acute or chronic administration of 10 nm or 50 nm GNPs could alter the liver, kidney functions and blood profile on day 7 post-treatment, however, these values returned to the normal levels on day 60 post- injection. Also, the chronic administration of GNPs induced a hypoglycemic effect in male albino rats.

中文翻译:

施用10 nm或50 nm金纳米颗粒(AuNPs)对雄性白化病大鼠的血型,肝肾功能的影响

这项工作旨在调查金纳米颗粒(GNP)的急性和慢性给药对雄性白化病大鼠肝和肾功能,血糖浓度,脂质分布和血液学参数的影响。进行了两个实验。在急性研究中:54只成年雄性大鼠随机分为三组,每组18只。第1组(对照组):其中大鼠肌肉内(im)注射1ml 0.9%生理盐水。第2组(50 nm GNP组):大鼠单次注射75 µg 50 nm GNP / kg体重(bwt)。在第3组(10 nm GNPs组)中:大鼠以75 µg 10 nm GNPs / kg bwt的单次剂量注射。在长期研究中:将18只成年雄性大鼠随机分为三组,每组6只。І组(对照组):大鼠为肌肉注射(i。m)每星期一次注射1 ml 0.9%的生理盐水,每周5次,持续数周。第2组(50 nm GNP):每星期一次给大鼠注射75 µg 50 nm GNP / kg bwt的剂量,持续5周。在第3组(10 nm GNP)中:雄性大鼠每周一次注射75 µg 50 nm GNP / kg bwt的剂量,持续5周,然后所有组的冲洗期为3周。在急性实验中,在第3、7和60天收集血液,而在慢性实验中,仅在2个月之前和之后收集血液。在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后第7天,用GNPs处理的大鼠表现出明显的(9%一次/每周5个星期。第2组(50 nm GNP):每星期一次给大鼠注射75 µg 50 nm GNP / kg bwt的剂量,持续5周。在第3组(10 nm GNP)中:雄性大鼠每周一次注射75 µg 50 nm GNP / kg bwt的剂量,持续5周,然后所有组的冲洗期为3周。在急性实验的第3、7和60天收集血液,而在慢性实验的2个月之前和之后才收集血液。在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后第7天,用GNPs处理的大鼠表现出明显的(9%一次/每周5个星期。第2组(50 nm GNP):每星期一次给大鼠注射75 µg 50 nm GNP / kg bwt的剂量,持续5周。在第3组(10 nm GNP)中:雄性大鼠每周一次注射75 µg 50 nm GNP / kg bwt的剂量,持续5周,然后所有组的冲洗期为3周。在急性实验中,在第3、7和60天收集血液,而在慢性实验中,仅在2个月之前和之后收集血液。在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后第7天,用GNPs处理的大鼠表现出明显的(每周注射一次,剂量为75 µg 50 nm GNP / kg bwt),持续5周。在第3组(10 nm GNP)中:雄性大鼠每周一次注射75 µg 50 nm GNP / kg bwt的剂量,持续5周,然后所有组的冲洗期为3周。在急性实验中,在第3、7和60天收集血液,而在慢性实验中,仅在2个月之前和之后收集血液。在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后第7天,用GNPs处理的大鼠表现出明显的(每周注射一次,剂量为75 µg 50 nm GNP / kg bwt),持续5周。在第3组(10 nm GNP)中:雄性大鼠每周一次注射75 µg 50 nm GNP / kg bwt的剂量,持续5周,然后对所有组进行3周的冲洗期。在急性实验中,在第3、7和60天收集血液,而在慢性实验中,仅在2个月之前和之后收集血液。在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后第7天,用GNPs处理的大鼠表现出明显的(每周一次以75 µg 50 nm GNPs / kg bwt的剂量注射5周,然后所有组的冲洗期为3周。在急性实验中,在第3、7和60天收集血液,而在慢性实验中,仅在2个月之前和之后收集血液。在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后的第7天,用GNPs治疗的大鼠表现出明显的(每周一次以75 µg 50 nm GNPs / kg bwt的剂量注射5周,然后所有组的冲洗期为3周。在急性实验中的第3、7和60天收集血液,而在慢性实验中仅在2个月之前和之后收集血液。在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后第7天,用GNPs处理的大鼠表现出明显的(在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后第7天,用GNPs处理的大鼠表现出明显的(在研究的第3天和第60天,雄性白化病大鼠的急性和慢性GNPs(10或50 nm大小)未引起肝肾功能,脂质谱参数和不同血液学参数的显着改变。但是,在治疗后第7天,用GNPs处理的大鼠表现出明显的(P <0.05)血清ALT,AST,ALP,尿素,肌酐,葡萄糖和不同血脂水平升高,HDL水平降低。长期服用10 nm或50 nm GNPs(P <0.05)会降低血清葡萄糖水平。总之,在治疗后第7天,急性或慢性给予10 nm或50 nm GNP可能会改变肝,肾功能和血液分布,但是,这些值在注射后60天恢复到正常水平。而且,长期施用GNPs在雄性白化病大鼠中引起降血糖作用。
更新日期:2020-07-28
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