The bark of Terminilia Arjuna is known for its numerous health benefits in traditional system of medicine. Nanotechnology based delivery system have added advantages of action at targeted location and improved cellular uptake. In the present paper, polymeric nanoparticles of methanolic extract of Terminilia Arjuna (Arjun ki chhal) were synthesized by using solvent evaporation method. The synthesized polymeric nanoparticles were further characterized using SEM, TEM, XRD, FTIR followed by evaluation of antimicrobial activity. The SEM images showed spherical shaped nanoparticles. Further TEM images revealed that particles of size as small as 50-75 nm are formed. PLGA encapsulated nanoparticles shows entrapment efficiency as high as 96.8% and percentage yield comes out to be 45.3. Antimicrobial study using cup-plate method was carried out using two strains of gram (+) bacteria S. aureus and B. pumilus and two strains of gram (-) bacteria E. Coli and Pseudomonas aeruginosa which shows MIC at 2000 ppm for gram (+) bacteria (both S. aureus and B. pumilus) and 5000 ppm (E. coli) and 6000 ppm (Pseudomonas aeruginosa) for gram (-) bacteria. From the results obtained it is proposed that polymeric nanoparticles were successfully formed which will enhance the efficacy of active components in the bark of Terminilia Arjuna and also demonstrates promising use in various pharmaceutical formulations as they show considerable results in the inhibition of bacterial growth.

中文翻译：

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负载阿朱那PLGA纳米颗粒的抗菌研究

Terminilia Arjuna的树皮因其在传统医学体系中的众多健康益处而闻名。基于纳米技术的输送系统增加了在目标位置的作用优势，并改善了细胞吸收。本文采用溶剂蒸发法合成了Terminilia Arjuna（Arjun ki chhal）甲醇提取物的聚合物纳米颗粒。使用SEM，TEM，XRD，FTIR进一步表征合成的聚合物纳米粒子，然后评估其抗菌活性。SEM图像显示球形纳米颗粒。进一步的TEM图像显示形成了小至50-75nm的颗粒。PLGA包封的纳米颗粒的包封率高达96.8％，产率百分比为45.3。使用两株革兰氏阳性菌（+）金黄色葡萄球菌和短杆菌（B. pumilus）以及两株革兰氏杆菌（-）的大肠杆菌（E. Coli）和铜绿假单胞菌（Pseudomonas aeruginosa）进行了杯碟法抗菌研究，其MIC为2000 ppm +）细菌（金黄色葡萄球菌和短小芽孢杆菌）以及革兰氏（-）细菌的5000 ppm（大肠杆菌）和6000 ppm（绿脓杆菌）。根据获得的结果，提出成功地形成了聚合物纳米颗粒，其将增强Terminilia Arjuna的树皮中活性成分的功效，并且由于它们在抑制细菌生长方面显示出可观的结果，因此也证明了其在各种药物制剂中的应用前景。大肠杆菌和铜绿假单胞菌对革兰氏阳性细菌（金黄色葡萄球菌和短小芽孢杆菌）的MIC为2000 ppm，而对革兰氏（-）细菌则为5000 ppm（大肠杆菌）和6000 ppm（铜绿假单胞菌）。根据获得的结果，提出成功地形成了聚合物纳米颗粒，其将增强Terminilia Arjuna的树皮中活性成分的功效，并且由于它们在抑制细菌生长方面显示出可观的结果，因此也证明了其在各种药物制剂中的应用前景。大肠杆菌和铜绿假单胞菌对革兰氏阳性细菌（金黄色葡萄球菌和短小芽孢杆菌）的MIC为2000 ppm，而对革兰氏（-）细菌则为5000 ppm（大肠杆菌）和6000 ppm（铜绿假单胞菌）。根据获得的结果，提出成功地形成了聚合物纳米颗粒，其将增强Terminilia Arjuna的树皮中活性成分的功效，并且由于它们在抑制细菌生长方面显示出可观的结果，因此也证明了其在各种药物制剂中的应用前景。