Honey is a natural product collected from the nectar by honey bees and has a wide array of therapeutic important compounds called as phytochemicals. Cancer is one of the leading causes of death worldwide. Existing conventional cancer therapy which is mainly comprised of chemotherapeutic agents has side effects. Honey due to the presence of phytochemicals especially flavonoids and phenolic acids like apigenin, chrysin, quercetin and caffeic acid are found to inhibit the cancer growth. This effect varies among different honeys since they are dependent on the phytochemical composition and their concentrations in honey, which varies with respect to floral origin from which honey has been collected, geographical and climatic conditions. Thus, in the current study, phytochemical characterization of Jambhul, Rubber, Litchi and Drumstick honey samples along with their effect on the expression of proteins playing a vital role in the event of cell cycle and apoptosis i.e. Cyclin B1 and p53 proteins were determined by Flow cytometry. It was found that these honey samples contain apigenin, chrysin, quercetin and caffeic acid. These honeys exhibited an increase in expression of p53 whereas, expression of Cyclin B1 protein was reduced in HeLa cells treated with honey samples. The presence of phytochemical markers exhibiting promising anticancer potential along with the ability of alterations in the expression of these proteins proves that these honey samples have a potent anticancer activity which enhances the chances of these Indian honeys to be utilized in the treatment of cervical cancer.

中文翻译：

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印度蜂蜜对HeLa细胞中p53和cyclin B1表达的影响

蜂蜜是蜜蜂从花蜜中提取的天然产物，并具有多种治疗上重要的化合物，称为植物化学物质。癌症是全球死亡的主要原因之一。现有的主要由化学治疗剂组成的常规癌症疗法具有副作用。蜂蜜由于存在植物化学物质，尤其是类黄酮和酚酸，如芹菜素，菊花素，槲皮素和咖啡酸而被发现可以抑制癌症的生长。由于不同的蜂蜜取决于植物化学成分及其在蜂蜜中的浓度，因此这种效应会有所不同，蜂蜜的浓度随花蜜的来源，地理和气候条件而变化。因此，在当前的研究中，Jambhul，Rubber，荔枝和鸡腿蜂蜜样品及其对蛋白质表达的影响，这些蛋白质在细胞周期和细胞凋亡的事件中起着至关重要的作用，即流式细胞仪测定了Cyclin B1和p53蛋白。发现这些蜂蜜样品中含有芹菜素，菊花素，槲皮素和咖啡酸。这些蜂蜜显示出p53表达的增加，而在用蜂蜜样品处理的HeLa细胞中，Cyclin B1蛋白的表达降低了。植物化学标记物显示出有希望的抗癌潜力以及这些蛋白质表达改变的能力，证明这些蜂蜜样品具有有效的抗癌活性，从而增加了这些印度蜂蜜在宫颈癌治疗中的机会。