Mitomycin-C (MC), an anticancer agent, induces oxidative stress in normal tissues causing severe toxicity. The present study aimed to evaluate whether the side effects of MC may be diminished by the incorporation of MC into nanoemulsion containing Roman chamomile oil (RCM-NE). Fifty mice were equally divided into five groups. The first and second groups were the control and the untreated Ehrlich ascites carcinoma bearing mice (EAC), respectively, while the other three groups were EAC-bearing mice treated once intraperitoneally with a dose of 200 µL of RCM-NE, 1 mg/kg⁻¹ MC/200 µL normal saline, and 1 mg/kg⁻¹ MC/200 µL RCM-NE. The protective effect of the RCM-NE was examined by measuring the alterations in complete blood count, organs weight indices, and levels of serum biochemical parameters. The oxidative stress was assessed by measuring lipid peroxidation and enzyme activities of catalase, superoxide dismutase, glutathione reductase, and thioredoxin reductase-1. MC-RCM-NE has significantly reduced the toxicity of MC on the white blood cells and platelets. It improved the renal and cardiac biomarker levels and the antioxidants of the kidney, heart, and thymus. The protective effect of the RCM-NE on the blood and organs against MC toxicity was associated with the decreasing of oxidative stress and maintaining the activity of the antioxidants.

中文翻译：

---

含罗马甘菊油的纳米乳剂对丝裂霉素C致埃氏腹水癌小鼠毒性的保护作用

丝裂霉素-C（MC）是一种抗癌剂，可在正常组织中诱导氧化应激，从而引起严重的毒性。本研究旨在评估是否可以通过将MC掺入含有罗马甘菊油（RCM-NE）的纳米乳液中来减轻MC的副作用。将五十只小鼠平均分为五组。第一组和第二组分别是对照组和未经治疗的携带Ehrlich腹水的小鼠（EAC），而其他三组是带有EAC的小鼠，腹腔内一次以200 µL RCM-NE，1 mg / kg的剂量治疗^-1 MC / 200 µL生理盐水和1 mg / kg ^-1MC / 200 µL RCM-NE。通过测量全血细胞计数，器官重量指数和血清生化参数水平的变化来检查RCM-NE的保护作用。通过测量脂质过氧化和过氧化氢酶，超氧化物歧化酶，谷胱甘肽还原酶和硫氧还蛋白还原酶-1的酶活性来评估氧化应激。MC-RCM-NE大大降低了MC对白细胞和血小板的毒性。它改善了肾脏和心脏的生物标志物水平以及肾脏，心脏和胸腺的抗氧化剂。RCM-NE对血液和器官抵抗MC毒性的保护作用与氧化应激的降低和维持抗氧化剂的活性有关。