The expression of transient receptor potential protein channels increases intensively colon and breast cancer cells. We aimed to reveal the role of 5-fluorouracil (5FU) and capecitabine along with Transient Receptor Potential Vanilloid 1 (TRPV1) channels in breast and colon cancer cells. Breast (MCF-7) and colon (Caco-2) cells were cultured and the study was planned as 7 main groups. Cells in the group were incubated with 5FU and capecitabine for 24 h and then incubated with TRPV1 channel antagonist capsazepine and stimulator capsaicin. The effects of medicines were investigated on molecular pathways of apoptosis. It was concluded that the administration of TRPV1 channel stimulator capsaicin in both cancer cells significantly increased the degree of intracellular Ca²⁺ levels and apoptosis levels compared to the control group whereas, the use of TRPV1 channel inhibitor capsazepine, significantly decreased the degree of apoptosis levels. The apoptotic effects of 5FU and capecitabine on both colon and breast cancer cells are directly related to TRPV1 channels and TRPV1 channels play an important role in the apoptosis. The apoptotic cell lines activity of capecitabine was higher in breast cancer cells, while that of 5FU was more pronounced in colon cancer cells.

中文翻译：

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卡培他滨和5-氟尿嘧啶通过TRPV1通道对人癌细胞的凋亡效率

瞬时受体潜在蛋白通道的表达大量增加结肠和乳腺癌细胞。我们旨在揭示5-氟尿嘧啶（5FU）和卡培他滨以及瞬时受体潜在香草酸1（TRPV1）通道在乳腺癌和结肠癌细胞中的作用。培养了乳腺癌（MCF-7）和结肠（Caco-2）细胞，并将该研究计划为7个主要组。将该组细胞与5FU和卡培他滨一起孵育24小时，然后与TRPV1通道拮抗剂辣椒素和刺激辣椒素一起孵育。研究了药物对细胞凋亡的分子途径的影响。结论是，在两个癌细胞中施用TRPV1通道刺激物辣椒素均显着增加了细胞内Ca ^2+的表达与对照组相比，TRPV1水平和细胞凋亡水平高，而使用TRPV1通道抑制剂卡塞平则显着降低了细胞凋亡水平。5FU和卡培他滨对结肠癌细胞和乳腺癌细胞的凋亡作用均与TRPV1通道直接相关，TRPV1通道在细胞凋亡中起重要作用。卡培他滨的凋亡细胞系活性在乳腺癌细胞中较高，而5FU在结肠癌细胞中则更为明显。