The bZIP gene is a transcription factor that plays various roles in relation to plant stress and hormone signaling. This gene is also involved in plant environmental stress and herbicide tolerance. We generated Nicotiana benthamiana transgenic plants with LebZIP2-encoding gene isolated from tomatoes using Agrobacterium-mediated transformation. Transgenic seeds harvested from these T₀ transgenic plants were grown and examined for gene transfer and changes in phenolic compounds in the T₁ generation. RT-PCR analysis using a primer specific to the LebZIP gene confirmed that the gene was transferred to the T₁ generation. We analyzed the increase and decrease tendency for 30 phenolic compounds using the T₁ generation-transgenic plants and investigated the mechanism between the specifically increased compound and LebZIP2 gene. Gallic acid, homogentisic acid, protocatechuic acid, myricetin, t-cinnamic acid, and b-resorcyclic acid were identified as the phenolic compounds that increased in T₁ transgenic plants overexpressing the LebZIP gene. Among these, homogentisic acid at 246.75-1055.19 µg/g, was increased by 2-5 fold in the T₁ transgenic plants compared to the control. Protocatechuic acid was found at 1640.54-2456.00 µg/g and was increased by 2-4 fold in T₁ transgenic plants. t-Cinnamic acid was present in a small amount of 23.14 µg/g in the control, whereas it was 102.19-135.47 µg/g in T₁ transgenic plants, showing an increase of 4-5 folds. These results indicated that homogentisic acid, protocatechuic acid, and t-cinnamic acid among the 30 phenolic compounds analyzed, were significantly increased in LebZIP2-overexpressing T₁ transgenic plants, and support the evidence that the LebZIP2 gene is significantly involved in the increment of three phenolic compounds.

中文翻译：

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过量表达LebZIP2的转基因植物中酚类化合物的变化

bZIP基因是一种转录因子，在植物胁迫和激素信号传导方面起着多种作用。该基因还参与植物环境胁迫和除草剂耐受性。我们使用农杆菌介导的转化方法，从番茄中分离出了LebZIP2编码基因，从而产生了本氏烟草转基因植物。生长从这些T ₀转基因植物中收获的转基因种子，并检查T ₁代中的基因转移和酚类化合物的变化。使用对LebZIP基因具有特异性的引物进行RT-PCR分析，证实该基因已转移至T ₁世代。我们使用T分析了30种酚类化合物的增加和减少趋势_研究了1代转基因植物，并研究了特异性增加的化合物与LebZIP2基因之间的机制。没食子酸，高纯酸，原儿茶酸，杨梅素，t-肉桂酸和b-间苯二酸被确定为在过表达LebZIP基因的T ₁转基因植物中增加的酚类化合物。其中，与对照相比，T ₁转基因植物中的高纯酸为246.75-1055.19 µg / g，增加了2-5倍。发现原儿茶酸为1640.54-2456.00 µg / g，在T ₁转基因植物中增加了2-4倍。对照中叔肉桂酸的含量为23.14 µg / g，而T _1中的含量为102.19-135.47 µg / g。转基因植物，显示增加4-5倍。这些结果表明所分析的30种酚类化合物中的高纯酸，原儿茶酸和t-肉桂酸在过表达LebZIP2的T ₁转基因植物中显着增加，并支持LebZIP2基因显着参与了3种植物的增量酚类化合物。