The aim of this study has been to fabricate a hybrid electrospun nanofibrous scaffold composed of poly(lactic-co-glycolic) acid (PLGA)/fibrin polymers to be used as a skin substitute and analyze its physical and biological properties. Fibrin was obtained from rat blood plasma, characterized and solubilized in formic acid. The final electrospinning solution concentration was 40% PLGA (w/v) and 1% fibrin (w/v). To improve spinnability, 3% PEG (w/v) was added. The scaffolds were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Water contact angle, maximum elongation, thermal stability, degree of swelling, blood compatibility, cytotoxicity and cell viability were analyzed. The characterization by SEM showed randomly oriented nanofibers with a mean diameter of 639.8 ± 241.8 nm for the PLGA/fibrin and 1051.0 ± 290.2 nm for the PLGA. FTIR spectra confirmed the presence of fibrin in the mats. Fibrin incorporation reduced the water c...

中文翻译：

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开发纤维状 PLGA/纤维蛋白支架作为潜在的皮肤替代品。

本研究的目的是制造由聚（乳酸-乙醇酸共聚物）/纤维蛋白聚合物组成的混合电纺纳米纤维支架，用作皮肤替代品，并分析其物理和生物特性。纤维蛋白从大鼠血浆中获得，表征并溶解在甲酸中。最终的静电纺丝溶液浓度为 40% PLGA (w/v) 和 1% 纤维蛋白 (w/v)。为了提高可纺性，添加了 3% PEG (w/v)。通过扫描电子显微镜 (SEM) 和傅里叶变换红外光谱 (FTIR) 对支架进行表征。分析了水接触角、最大伸长率、热稳定性、溶胀度、血液相容性、细胞毒性和细胞活力。SEM 表征显示随机取向的纳米纤维平均直径为 639.8 ± 241。PLGA/纤维蛋白为 8 nm，PLGA 为 1051.0 ± 290.2 nm。FTIR 光谱证实了垫子中存在纤维蛋白。纤维蛋白的掺入减少了水...