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A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A.
Analyst ( IF 3.6 ) Pub Date : 2020-07-27 , DOI: 10.1039/d0an01220c Yangjun Ding 1 , Hezhen Shang , Xiaokun Wang , Lingxin Chen
Analyst ( IF 3.6 ) Pub Date : 2020-07-27 , DOI: 10.1039/d0an01220c Yangjun Ding 1 , Hezhen Shang , Xiaokun Wang , Lingxin Chen
Affiliation
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Ochratoxin A (OTA) is a well-known carcinogenic contaminant in food commodities. The highly sensitive and specific detection of the OTA level is an essential way to prevent the health risks to humans/animals. In this work, an SERS-based competitive immunoassay platform has been developed for the detection of OTA. This assay contains two compartments: OTA-BSA-immobilized SERS nanotags and anti-OTA antibody-functionalized magnetic beads. In the presence of target OTA, a competitive reaction towards magnetic beads occurs between the target OTA and SERS nanotags. The characteristic peaks of SERS nanotags adsorbed onto magnetic beads were used for the quantitative analysis of OTA. Under optimized conditions, a good linear relationship was obtained in the range of 1 pg mL−1 to1000 pg mL−1. The limit of detection (LOD) was estimated to be 0.61 pg mL−1 using the IUPAC standard method. In addition, this proposed method was also successfully applied for the detection of OTA in wine samples and satisfactory recovery rates were obtained in the range of 90.60% to 103.3%. It is believed that this SERS-based competitive method holds great potential for highly sensitive and specific detection of mycotoxins.
中文翻译：
基于SERS的竞争性免疫测定法，用于highly曲霉毒素A的高灵敏度和特异性检测。
ch曲毒素A（OTA）是食品中众所周知的致癌污染物。对OTA水平的高度敏感和特异的检测是预防对人类/动物健康的重要手段。在这项工作中，已经开发了基于SERS的竞争性免疫分析平台来检测OTA。该测定法包含两个区室：OTA-BSA固定的SERS纳米标签和抗OTA抗体功能化的磁珠。在靶标OTA的存在下，在靶标OTA和SERS纳米标签之间发生了对磁珠的竞争反应。吸附在磁珠上的SERS纳米标签的特征峰用于OTA的定量分析。在优化条件下，在1 pg mL -1至1000 pg mL -1的范围内获得了良好的线性关系。使用IUPAC标准方法，检测下限（LOD）估计为0.61 pg mL -1。此外，该方法也成功地用于葡萄酒样品中OTA的检测，回收率在90.60％至103.3％之间，令人满意。可以相信，这种基于SERS的竞争方法在高度敏感和特异性检测霉菌毒素方面具有巨大潜力。
更新日期：2020-09-14
中文翻译：
基于SERS的竞争性免疫测定法，用于highly曲霉毒素A的高灵敏度和特异性检测。
ch曲毒素A（OTA）是食品中众所周知的致癌污染物。对OTA水平的高度敏感和特异的检测是预防对人类/动物健康的重要手段。在这项工作中，已经开发了基于SERS的竞争性免疫分析平台来检测OTA。该测定法包含两个区室：OTA-BSA固定的SERS纳米标签和抗OTA抗体功能化的磁珠。在靶标OTA的存在下，在靶标OTA和SERS纳米标签之间发生了对磁珠的竞争反应。吸附在磁珠上的SERS纳米标签的特征峰用于OTA的定量分析。在优化条件下，在1 pg mL -1至1000 pg mL -1的范围内获得了良好的线性关系。使用IUPAC标准方法，检测下限（LOD）估计为0.61 pg mL -1。此外，该方法也成功地用于葡萄酒样品中OTA的检测，回收率在90.60％至103.3％之间，令人满意。可以相信，这种基于SERS的竞争方法在高度敏感和特异性检测霉菌毒素方面具有巨大潜力。
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