Here we applied target analysis to a temperature dependent flash photolysis dataset of the light-driven sodium ion pump Krokinobacter rhodopsin 2 (KR2) at sodium pumping conditions. With an increase in temperature from 10 - 40 °C, the overall photocycle duration was accelerated by a factor of six, while single transitions like the L to M transition increased by a factor of 40. Using kinetic modeling with the Eyring constraint as well as spectral corrections on the datasets the spectral position as well as the equilibria of the different photointermediates could be resolved. The results provide further insight into KR2s photocycle and energetics.

中文翻译：

---

Krokinobacter rhodopsin 2动力学的温度依赖性

在这里，我们将目标分析应用于在钠泵浦条件下光驱动钠离子泵Krokinobacter rhodopsin 2（KR2）的温度依赖性闪光光解数据集。随着温度从10-40°C升高，整个光循环持续时间加快了6倍，而从L到M的单个跃迁则增加了40倍。使用带有Eyring约束的动力学模型以及数据集上的光谱校正可以解决不同光中间体的光谱位置以及平衡问题。结果为KR2的光周期和能量学提供了进一步的见解。