Abstract

Here we report the identification and quantitation of cyclopropyl-fentanyl in a fatality casework occurred due to a poly-drug toxicity in Spain in December 2017. The cyclopropyl-fentanyl was identified in non-biological (paraphernalia) and biological samples (whole-blood, vitreous humor and urine). Conventional techniques (GC–MS) and the UV-Vis spectral allowed differencing between the two structural isomer compounds of fentanyl (cyclopropyl and crotonyl) when the CRM was not available at the laboratory. Both of the drugs have the same MS spectra but different UV-Vis spectra due to the presence of an additional chromophore group in the case of crotonyl. The cyclopropyl-fentanyl detection allowed generating an alert and contributing to the surveillance and detection of this dangerous substance found mixed in doses of clandestine sale heroin. Then, high-resolution analytical techniques (LC–HRMS-MS), showed limitations for the identification of the isobaric fentanyl compounds but they had a high potential for fentanyl metabolites identification. Two tentative metabolites were identified in urine samples: cyclopropyl-norfentanyl and N-methyl cyclopropyl-norfentanyl. Finally, the systematic routine method (LC–MS-MS) was validated and applied to the quantification of cyclopropyl-fentanyl in a blood sample. A obtained value (20.4 ng/mL) was in the range of those reported in other cases in different countries (from 8 to 30 mg/ mL), being the determined concentration of cyclopropyl-fentanyl high enough to infer that this fentanyl analog had a main role as cause of death. As far as we know, this is the first fatality reported in Spain involving cyclopropyl-fentanyl

中文翻译：

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紫外可见和高分辨率质谱法在西班牙首例致命病例中鉴定环丙基-芬太尼。

摘要

在这里，我们报告了由于多药毒性于2017年12月在西班牙造成的死亡案例中，对环丙基-芬太尼的鉴定和定量分析。在非生物（用具）和生物样品（全血，玻璃体液和尿液）。当实验室中没有CRM时，常规技术（GC-MS）和UV-Vis光谱可以区分芬太尼的两种结构异构体化合物（环丙基和巴豆酰基）。由于在巴豆酰基的情况下，由于存在另外的生色团基团，因此两种药物的MS光谱相同，但UV-Vis光谱不同。环丙基-芬太尼的检测可以产生警报，并有助于监视和检测混合在秘密销售海洛因剂量中的这种危险物质。然后，高分辨率分析技术（LC-HRMS-MS）显示出异丁酸芬太尼化合物鉴定的局限性，但它们具有较高的芬太尼代谢物鉴定潜力。在尿液样品中鉴定出两种试验性代谢物：环丙基-去甲芬太尼和N-甲基环丙基-去甲芬太尼。最后，验证了系统常规方法（LC-MS-MS）并将其用于定量血液样品中的环丙基-芬太尼。所获得的值（20.4 ng / mL）在不同国家/地区的其他情况下报告的范围内（从8至30 mg / mL），是确定的环丙基-芬太尼浓度，其高至足以推断该芬太尼类似物具有作为死亡原因的主要作用。据我们所知，这是西班牙报道的首例涉及环丙基-芬太尼的死亡