Abstract

The emerging paradigm in the immunology of pregnancy is that implantation of conceptuses does not progress in an immunologically suppressed environment. Rather, the endometrium undergoes a controlled inflammatory response during implantation as trophectoderm of elongating and implanting pig conceptuses secrete the pro-inflammatory cytokine interferon gamma (IFNG). Results of this study with pigs revealed: (1) accumulation of immune cells and apoptosis of stromal cells within the endometrium at sites of implantation during the period of IFNG secretion by conceptuses; (2) accumulation of proliferating cell nuclear antigen (PCNA)-positive T cells within the endometrium at sites of implantation; (3) significant increases in expression of T cell co-signaling receptors including programmed cell death 1 (PDCD1), CD28, cytotoxic T-lymphocyte associated protein 4 (CTLA-4), and inducible T cell co-stimulator (ICOS), as well as chemokines CXCL9, 10, and 11 within the endometrium at sites of implantation; (4) significant increases in T cell co-signaling receptors, PDCD1 and ICOS, and chemokine CXCL9 in the endometrium of cyclic gilts infused with IFNG; and (5) identification of CD4⁺ (22.59%) as the major T cell subpopulation, with minor subpopulations of CD8⁺ (1.38%), CD4⁺CD25⁺ (1.08%), and CD4⁺CD8⁺ (0.61%) T cells within the endometrium at sites of implantation. Our results provide new insights into the immunology of implantation to suggest that trophectoderm cells of pigs secrete IFNG to recruit various subpopulations of T cells to the endometrium to contribute to a controlled inflammatory environment that supports the active breakdown and restructuring of the endometrium in response to implantation of the conceptus.

中文翻译：

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在围着床期间，猪概念分泌干扰素伽马以将 T 细胞募集到子宫内膜。

摘要

妊娠免疫学中的新兴范式是，在免疫抑制的环境中，胚胎的植入不会进展。相反，子宫内膜在着床期间经历受控的炎症反应，因为延长和着床猪受孕的滋养外胚层分泌促炎细胞因子干扰素γ（IFNG）。这项对猪的研究结果表明：（1）受孕者分泌IFNG期间子宫内膜内免疫细胞的积累和基质细胞的凋亡；(2) 增殖细胞核抗原 (PCNA) 阳性 T 细胞在植入部位子宫内膜内的积累；(3) T 细胞共信号受体的表达显着增加，包括程序性细胞死亡 1 (PDCD1)、CD28、细胞毒性 T 淋巴细胞相关蛋白 4 (CTLA-4) 和诱导性 T 细胞共刺激剂 (ICOS)，以及植入部位子宫内膜内的趋化因子 CXCL9、10 和 11；(4) 注入IFNG的循环小母猪子宫内膜中T细胞共信号受体PDCD1和ICOS以及趋化因子CXCL9显着增加；(5) CD4的鉴定⁺ (22.59%) 作为主要 T 细胞亚群，在植入部位的子宫内膜内有少量 CD8 ⁺ (1.38%)、CD4 ⁺ CD25 ⁺ (1.08%) 和 CD4 ⁺ CD8 ⁺ (0.61%) T 细胞亚群. 我们的研究结果提供了对着床免疫学的新见解，表明猪的滋养外胚层细胞分泌 IFNG 以将各种 T 细胞亚群募集到子宫内膜，从而促进受控的炎症环境，支持子宫内膜对着床作出反应的主动分解和重组的概念。