Abstract

The two-way communication between the mother and the fetus is accomplished by immune cells. CD8+ T cells of normal pregnant (NP) women express progesterone receptor (PR). Binding of PR to progesterone (P) and the production of progesterone-induced blocking factor (PIBF) can aid immune escape, which is an important factor in the maternal immune response. We detected the proportion of CD8+ T cells and the expression of the surface costimulatory molecules BTLA, TIGIT, ICOS, and PD-1 in peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion (URSA) and in NP women. All patients were at 8 -10 weeks of gestation. The results showed that there was no change in the proportions of CD8+ T cells in peripheral blood and decidual tissues of URSA patients compared to those of NP women. In peripheral blood, compared with the NP group, the URSA group showed decreased expression of BTLA + CD8+ T cells and the difference was statistically significant, but there was no difference between the groups in terms of TIGIT + CD8+, PD-1 + CD8+, and ICOS + CD8+ T cells. There was no change in the levels of TIGIT + CD8+, PD-1 + CD8+, ICOS + CD8+, and BTLA + CD8+ T cells in decidual tissue. These data confirm that the number of CD8+ T cells in peripheral blood and decidual tissue is not the main factor leading to the pathogenesis of URSA, and other immune cells may play an important role in URSA, but this hypothesis needs further exploration and research.

中文翻译：

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不明原因复发性流产妇女外周血和蜕膜CD8+T细胞BTLA、ICOS、PD-1和TIGIT表达的明显变化。

摘要

母亲和胎儿之间的双向交流是由免疫细胞完成的。正常怀孕 (NP) 妇女的 CD8+ T 细胞表达孕酮受体 (PR)。PR 与孕酮 (P) 的结合和孕酮诱导阻断因子 (PIBF) 的产生可以帮助免疫逃逸，这是母体免疫反应的一个重要因素。我们检测了不明原因复发性自然流产 (URSA) 女性和 NP 女性的外周血和蜕膜组织中 CD8+ T 细胞的比例和表面共刺激分子 BTLA、TIGIT、ICOS 和 PD-1 的表达。所有患者均在妊娠 8 -10 周。结果显示，与NP女性相比，URSA患者外周血和蜕膜组织中CD8+T细胞的比例没有变化。在外周血中，与NP组相比，URSA组BTLA+CD8+T细胞表达降低，差异有统计学意义，但TIGIT+CD8+、PD-1+CD8+、ICOS+CD8+组间无差异T细胞。蜕膜组织中TIGIT+CD8+、PD-1+CD8+、ICOS+CD8+和BTLA+CD8+T细胞水平无变化。这些数据证实，外周血和蜕膜组织中CD8+T细胞的数量并不是导致URSA发病的主要因素，其他免疫细胞可能在URSA中起重要作用，但这一假设还需要进一步探索和研究。和 ICOS + CD8+ T 细胞。蜕膜组织中TIGIT+CD8+、PD-1+CD8+、ICOS+CD8+和BTLA+CD8+T细胞水平无变化。这些数据证实外周血和蜕膜组织中CD8+T细胞的数量不是导致URSA发病的主要因素，其他免疫细胞可能在URSA中起重要作用，但这一假设还需要进一步探索和研究。和 ICOS + CD8+ T 细胞。蜕膜组织中TIGIT+CD8+、PD-1+CD8+、ICOS+CD8+和BTLA+CD8+T细胞水平无变化。这些数据证实，外周血和蜕膜组织中CD8+T细胞的数量并不是导致URSA发病的主要因素，其他免疫细胞可能在URSA中起重要作用，但这一假设还需要进一步探索和研究。