当前位置: X-MOL 学术Geomicrobiol. J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Biosolubilization of Low-Grade Rock Phosphate by Native Microbial Consortia from Phosphate Mines: Effect of Sampling Sources and Culture Media
Geomicrobiology Journal ( IF 2.2 ) Pub Date : 2020-07-27 , DOI: 10.1080/01490451.2020.1793033
Chunqiao Xiao 1 , Yan Zhou 1 , Jingang Hu 1 , Shuyu Guo 1 , Na Zhou 1 , Ruan Chi 2
Affiliation  

Abstract Two native microbial consortia were isolated from the soil and drainage in the phosphate mines, and their abilities to solubilize low-grade rock phosphate (RP) in two different culture media, namely Pikovskaya (PKV) and National Botanical Research Institute’s phosphate (NBRIP) medium, respectively, were estimated. Results showed that the two microbial consortia could grow steadily in the solution and continuously released soluble phosphate from the RP during 14 d of experiments. This process was accompanied by a drop in pH of the solution. The microbial consortium isolated from the soil achieved the largest release of soluble phosphate and pH reduction in the PKV medium among the four microbial consortia named PS, NS, PD, and ND, respectively. Fourier transform infrared spectroscopy (FTIR) analysis of RP indicated that hydroxyl, alkyl C-H bond, amide, and carboxyl were involved in the RP solubilization, and significant changes of them were observed after the experiments. The four microbial consortia were sampled before and after the experiments for the analysis of their bacterial and fungal community structures by Illumina MiSeq sequencing. Results showed that the relative diversities and abundances of the dominant bacteria and fungi varied with different sampling sources (soil and drainage) and culture media (PKV and NBRIP medium).

中文翻译:

磷酸盐矿中原生微生物群落对低品位岩石磷酸盐的生物增溶作用:采样来源和培养基的影响

摘要 从磷矿的土壤和排水系统中分离出两种原生微生物聚生体,它们在两种不同的培养基中溶解低品位磷矿 (RP) 的能力,即 Pikovskaya (PKV) 和国家植物研究所的磷酸盐 (NBRIP)中,分别进行了估计。结果表明,在 14 d 的实验过程中,这两种微生物菌群在溶液中可以稳定生长,并从 RP 中持续释放可溶性磷酸盐。该过程伴随着溶液pH值的下降。在分别命名为 PS、NS、PD 和 ND 的四种微生物聚生体中,从土壤中分离出的微生物聚生体在 PKV 培养基中实现了最大的可溶性磷酸盐释放和 pH 降低。RP 的傅里叶变换红外光谱 (FTIR) 分析表明,羟基、烷基 CH 键、酰胺和羧基参与了 RP 的溶解,实验后观察到它们的显着变化。在实验前后对四种微生物聚生体进行采样,通过 Illumina MiSeq 测序分析其细菌和真菌群落结构。结果表明,优势细菌和真菌的相对多样性和丰度随采样来源(土壤和排水)和培养基(PKV和NBRIP培养基)的不同而不同。
更新日期:2020-07-27
down
wechat
bug