Abstract

Two compounds (7-O-methylmearnsitrin (7-OM) and roseoside A (RA) were identified and characterized from the leaves of Leea aequata (L. aequata) L. The cytotoxicity of 7-OM and RA on HeLa cells was performed using MTT. The 7-OM and RA showed significant inhibition of HeLa cell proliferation with an IC₅₀ of 22 and 20 µg/mL, respectively when compared with the standard vincristin sulphate (VS) (IC₅₀ of 15 µg/mL). Moreover, the 7-OM and RA significantly inhibit other cancer cells (HEK-293, H228, and H3122) when compared with the VS and the cytotoxic activity of the compounds might show through the induction of apoptosis. Strikingly, annexin-V and PI signals could barely be detected in control cells, while strong fluorescence densities were observed in response to treatment indicating that these compounds have capacity to induce HeLa cell apoptosis. Our results suggest that the anticancer activity of 7-OM and RA was due to the induction of apoptosis.

摘要

从Leea aequata ( L . aequata ) L的叶子中鉴定并表征了两种化合物( 7- O -methylmearnsitrin (7-OM)和蔷薇苷A (RA) 。使用7-OM和RA对HeLa细胞的细胞毒性MTT的7-OM和RA显示HeLa细胞增殖的抑制显著的IC ₅₀的22和20微克/毫升，分别在与标准长春新碱硫酸盐（VS）（IC相比₅₀15 微克/毫升）。此外，与 VS 相比，7-OM 和 RA 显着抑制其他癌细胞（HEK-293、H228 和 H3122），并且化合物的细胞毒活性可能通过诱导细胞凋亡来显示。引人注目的是，在对照细胞中几乎检测不到膜联蛋白-V 和 PI 信号，而在处理后观察到强荧光密度，表明这些化合物具有诱导 HeLa 细胞凋亡的能力。我们的结果表明 7-OM 和 RA 的抗癌活性是由于诱导细胞凋亡。