ABSTRACT

We investigated the wound healing effects of an ointment prepared from a hydroethanolic extract of Trifolium pratense (red clover) in Wistar rats. An animal model was prepared by making 314 mm² full thickness skin excisions in all animals. Rats were divided into four experimental groups. Group 1 was a non-intervention control. Groups 2, 3 and 4 were treated topically with ointments containing 1.5, 3.0 and 6% (w/v) T. pretense extract, respectively. We evaluated the effects of these ointments by histological analysis of collagen production, epidermis thickness, fibroblast distribution and wound contraction ratio, and also by estimating expression of Bax, Bcl-2 and p53. Collagen production, epidermis thickness, fibroblast distribution and wound contraction ratio were significantly increased in groups 2 − 4 compared to group 1. Groups 2 − 4 also exhibited significantly increased levels of Bcl-2 mRNA and reduced mRNA levels of Bax and p53 compared to group 1. Groups 2 − 4 exhibited accelerated healing by up-regulating Bcl-2 expression, reducing p53 and Bax levels, and by promoting the proliferative phase of healing. We suggest that a hydroethanolic extract of T. pratense in commercial ointments may be useful for promoting wound healing.

摘要

我们研究了从三叶草（红三叶草）的水乙醇提取物制备的软膏在 Wistar 大鼠中的伤口愈合效果。通过在所有动物中进行314mm ²全厚度皮肤切除来制备动物模型。将大鼠分成四个实验组。第 1 组是非干预对照。第 2、3 和 4 组用含有 1.5、3.0 和 6% (w/v) T. pretense 的软膏局部治疗分别提取。我们通过胶原蛋白生成、表皮厚度、成纤维细胞分布和伤口收缩率的组织学分析以及估计 Bax、Bcl-2 和 p53 的表达来评估这些软膏的效果。与第 1 组相比，第 2-4 组的胶原蛋白生成、表皮厚度、成纤维细胞分布和伤口收缩率显着增加。与第 1 组相比，第 2-4 组的 Bcl-2 mRNA 水平也显着增加，Bax 和 p53 的 mRNA 水平降低1. 第 2-4 组通过上调 Bcl-2 表达、降低 p53 和 Bax 水平以及促进愈合的增殖阶段表现出加速愈合。我们建议使用T. pratense的水乙醇提取物 在商业软膏中可能有助于促进伤口愈合。