Abstract Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s−1 and 40 s−1). For both strains and surfaces, biofilm development was higher at 4 s−1. Biofilm development of Nodosilinea sp. LEGE 06145 was substantially higher than Nodosilinea sp. LEGE 06119, but no significant differences were found between surfaces. Overall, 377 and 301 different proteins were identified for Nodosilinea sp. LEGE 06145 and Nodosilinea sp. LEGE 06119. Differences in protein composition were more noticeable in biofilms formed under different hydrodynamic conditions than in those formed on different surfaces. Ribosomal and photosynthetic proteins were identified in most conditions. The characterization performed gives new insights into how shear rate and surface affect the planktonic to biofilm transition, from a structural and proteomics perspective.

中文翻译：

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使用鸟枪蛋白质组学方法表征来自两种丝状蓝藻的浮游和生物膜细胞

摘要 蓝藻促进海洋生物污染并产生重大影响。通过 LC-MS/MS，对来自两种蓝藻的浮游生物和生物膜细胞进行了定性蛋白质组学分析。在海洋环境的两个相关流体动力学条件下（平均剪切速率为 4 s-1 和 40 s-1），在玻璃和有机玻璃上形成生物膜。对于菌株和表面，生物膜发展在 4 s-1 时更高。Nodosilinea sp. 的生物膜发育。LEGE 06145 显着高于 Nodosilinea sp。LEGE 06119，但在表面之间没有发现显着差异。总的来说，为 Nodosilinea sp. 鉴定了 377 和 301 种不同的蛋白质。LEGE 06145 和 Nodosilinea sp。LEGE 06119. 与在不同表面形成的生物膜相比，在不同流体动力学条件下形成的生物膜中蛋白质组成的差异更为明显。在大多数条件下都鉴定了核糖体和光合蛋白。从结构和蛋白质组学的角度，所进行的表征为剪切速率和表面如何影响浮游生物膜转变提供了新的见解。