Interleukin‐38 (IL‐38) is a novel member of the IL‐1 cytokine family with anti‐inflammatory activity. However, its effect on adipogenesis and inflammatory cytokines secretion of adipocytes in vitro has not been reported. To address whether IL‐38 inhibits adipogenesis and inflammation in vitro, adipose precursor 3T3‐L1 cells were cultured with or without IL‐38. The morphology and size of lipid droplets in 3T3‐L1 cells were measured by Oil red O staining. The mRNA expression levels of GATA‐binding protein‐3 (GATA‐3), glucose transporter type 4 (GLUT4), peroxisome proliferator‐associated receptor γ2, IL‐1β, IL‐6, and monocyte chemoattractant protein‐1 (MCP‐1) in 3T3‐L1 cells were detected by real‐time PCR, The contents of IL‐6, IL‐1β, and MCP‐1 in 3T3‐L1 cell medium supernatants were determined by enzyme‐linked immunosorbent assay. IL‐38 significantly decreased the number of lipid droplets in 3T3‐L1 cells. IL‐38 also increased GATA‐3 and GLUT4 mRNA expression and inhibited IL‐1β, IL‐6, and MCP‐1 secretion by 3T3‐L1 cells. It is concluded that IL‐38 can inhibit the differentiation of human adipocytes and inflammatory cytokine production by 3T3‐L1 cells.

中文翻译：

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白细胞介素38抑制3T3-L1前脂肪细胞中的脂肪生成和炎性细胞因子的产生。

白细胞介素38（IL-38）是IL-1细胞因子家族的新型成员，具有抗炎活性。然而，其在体外对脂肪形成和脂肪细胞炎性细胞因子分泌的影响尚未见报道。为了解决IL-38是否在体外抑制脂肪形成和炎症，在有或没有IL-38的情况下培养了脂肪前体3T3-L1细胞。通过油红O染色测量3T3-L1细胞中脂质滴的形态和大小。GATA结合蛋白3（GATA-3），4型葡萄糖转运蛋白（GLUT4），过氧化物酶体增殖物相关受体γ2，IL-1β，IL-6和单核细胞趋化蛋白1（MCP-1）的mRNA表达水平）通过实时PCR检测3T3-L1细胞中的3T3-L1细胞培养基上清液中IL-6，IL-1β和MCP-1的含量。IL-38显着减少了3T3-L1细胞中脂质滴的数量。IL-38还增加了GATA-3和GLUT4 mRNA的表达，并抑制3T3-L1细胞分泌IL-1β，IL-6和MCP-1。结论是IL-38可以抑制人脂肪细胞的分化和3T3-L1细胞产生的炎性细胞因子。