This work studies and models microbial inactivation by high hydrostatic pressure applied to insect protein (Acheta domesticus) enriched gels. The target microbial groups and pathogens studied were total aerobic mesophilic (TAM) bacteria, molds and yeasts (M&Y), Salmonella Typhimurium and Escherichia coli O157:H7. Results indicated that high hydrostatic pressure reduced the microbial load of both, the natural contaminating microorganisms (TAM and M&Y) and the intentionally inoculated pathogenic microorganisms. However, inactivation of the initial microbial load was lower than 5-log reductions. Kinetic inactivation parameters were deduced by using the Weibull distribution function which presented high goodness of fit. Kinetic parameters of the model showed that the most resistant microbial group was total aerobic mesophilic bacteria, followed by E. coli O157:H7, mold and yeast and Salmonella with kinetic parameters of 2.21, 1.58, 1.27, and 0.17 (min × mL/CFU) at 350 MPa, respectively. Regarding the pathogenic microorganisms inoculated in insect protein gels, E. coli O157:H7 may be a good candidate Gram negative target microorganism for high hydrostatic pressure process development, because it usually contaminates these products and was the one with the highest baro-resistance. These reasons support that high-pressure treatments for this type of food should be designed considering this microorganism as a target.

这项工作研究和建模高静水压力的微生物灭活作用于富含昆虫蛋白（Acheta domesticus）的凝胶。研究的目标微生物群和病原体是总需氧中温（TAM）细菌，霉菌和酵母菌（M＆Y），鼠伤寒沙门氏菌和大肠杆菌O157：H7。结果表明，高静水压力降低了天然污染微生物（TAM和M＆Y）和故意接种的病原微生物的微生物负荷。然而，初始微生物负荷的失活低于5-log的降低。通过使用威布尔分布函数推导了动力学失活参数，该函数具有很高的拟合度。该模型的动力学参数显示，最有抵抗力的微生物组是总需氧中温细菌，其次是大肠杆菌O157：H7，霉菌和酵母菌和沙门氏菌，动力学参数分别为2.21、1.58、1.27和0.17（min×mL / CFU ）分别在350 MPa下。关于接种在昆虫蛋白凝胶中的病原微生物，大肠杆菌O157：H7可能是用于高静水压工艺开发的革兰氏阴性目标微生物的良好候选者，因为它通常会污染这些产品，并且是耐压性最高的产品。这些原因支持应该以这种微生物为目标来设计用于此类食品的高压处理方法。