Abstract As virus spread can lead to severe epidemics and pandemics associated with high mortality, it is necessary to have a highly sensitive detection method for viruses. Although various detection methods have been developed so far, current methods in detecting a virus require preprocessing and involve quite intricate processes of low sensitivity. Here, we have developed a virus detection method with a broad dynamic range and high sensitivity, based on immuno-complex formation between quantum dot (QD)-embedded silica nanoparticles (QD2) and magnetic beads. The multiple QD- containing QD2s showed 500 times stronger photoluminescence than individual QDs. When biotin was immobilized as a ligand, streptavidin was detected in a range of 10 zM to 10 nM. The clinical applicability of the QD2-based system was examined using the avian virus (i.e., H1N1 influenza virus), and it showed a detection range of 4.76 × 10−4 ∼ 3.2 hemagglutination unit/mL. This result is comparable to the polymerase chain reaction method, and is approximately 2100 times more sensitive than the conventional hemagglutination method. Since the QD2-based system could detect target molecules with high sensitivity without requiring an amplification step, it can be applied in various biomedical and clinical fields.

中文翻译：

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基于高亮度量子点嵌入纳米探针和磁珠的宽动态范围病毒灵敏检测

摘要 由于病毒传播会导致严重的流行病和大流行病，死亡率很高，因此有必要对病毒进行高度灵敏的检测。尽管迄今为止已经开发了各种检测方法，但当前检测病毒的方法需要预处理并且涉及相当复杂的低灵敏度过程。在这里，我们开发了一种具有宽动态范围和高灵敏度的病毒检测方法，基于量子点 (QD) 嵌入的二氧化硅纳米粒子 (QD2) 和磁珠之间的免疫复合物形成。包含多个 QD 的 QD2 显示出比单个 QD 强 500 倍的光致发光。当生物素作为配体固定时，链霉亲和素的检测范围为 10 zM 至 10 nM。使用禽病毒检查了基于 QD2 的系统的临床适用性（即，H1N1 流感病毒），检测范围为 4.76 × 10−4 ∼ 3.2 血凝单位/mL。该结果可与聚合酶链反应法相媲美，并且比常规血凝法的灵敏度高约 2100 倍。由于基于 QD2 的系统可以在不需要放大步骤的情况下以高灵敏度检测目标分子，因此可以应用于各种生物医学和临床领域。