Generally, the selection of fructans prebiotics and probiotics for the formulation of a symbiotic has been based on arbitrary considerations and in vitro tests that fail to take into account competitiveness and other interactions with autochthonous members of the intestinal microbiota. However, such analyzes may be a valuable step in the development of the symbiotic. The present study, therefore, aims to investigate the effect of lactobacilli strains and fructans (prebiotic compounds) on the growth of the intestinal competitor Klebsiella oxytoca, and to assess the correlation with short-chain fatty acids production. The short-chain fatty acids formed in the fermentation of the probiotic/prebiotic combination were investigated using NMR spectroscopy, and the inhibitory activities were assessed by agar diffusion and co-culture methods. The results showed that Lactobacillus strains can inhibit K. oxytoca, and that this antagonism is influenced by the fructans source and probably associated with organic acid production.

通常，选择果聚糖益生元和益生菌用于共生制剂是基于任意考虑和体外测试，这些测试未考虑竞争性以及与肠道菌群自生成员的其他相互作用。但是，这种分析可能是共生发展中的重要一步。因此，本研究旨在研究乳酸杆菌菌株和果聚糖（益生元化合物）对肠道竞争者催产克雷伯菌的生长的影响，并评估与短链脂肪酸生产的相关性。使用NMR光谱研究了益生菌/益生元组合发酵中形成的短链脂肪酸，并通过琼脂扩散和共培养方法评估了抑制活性。结果表明，乳酸杆菌菌株可以抑制产氧假丝酵母，这种拮抗作用受果聚糖来源的影响，并可能与有机酸的产生有关。