Pythium oligandrum is an important potential biological control agent of plant pathogens. The objective of this study was to evaluate the genetic diversity and population structure of 45 isolates from various provinces in Iran. Genetic diversity was characterized based on inter-simple sequence repeat (ISSR) markers. Cluster analysis of ISSR data categorized the isolates into three main clusters. A high level of variability was revealed within P. oligandrum isolates, however, no significant diversity among geographical regions was observed and isolates from different regions were grouped into the same cluster. Genetic diversity was moderate within populations (i.e. Bushehr, Fars and Kermanshah) with high average gene flow and low genetic distances between populations. The observed and effective numbers of alleles were higher in the Fars population than in other studied populations. This is the first report on the genetic diversity of P. oligandrum using ISSR markers. This study paves the way for the selection of isolates to be assessed for their antagonistic efficacy.

中文翻译：

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伊朗寡枝腐霉的遗传多样性

Pythium oligandrum 是一种重要的潜在植物病原体生物防治剂。本研究的目的是评估来自伊朗各省的 45 个分离株的遗传多样性和种群结构。基于简单序列间重复 (ISSR) 标记表征遗传多样性。ISSR 数据的聚类分析将分离株分为三个主要聚类。在 P. oligandrum 分离株中揭示了高度的变异性，但是，在地理区域之间没有观察到显着的多样性，并且来自不同区域的分离株被分组到同一簇中。种群内（即 Bushehr、Fars 和 Kermanshah）的遗传多样性中等，平均基因流高，种群间遗传距离低。Fars 群体中观察到的等位基因的有效数量高于其他研究群体。这是第一份使用 ISSR 标记的 P. oligandrum 遗传多样性报告。该研究为选择分离株以评估其拮抗功效铺平了道路。