Bioactive carotenoids were isolated from different body components (head and carapace) of 3 types of shrimp (Fenneropenaeus indicus, Metapenaeus ensis, and Penaeus semisulcatus) using thin‐layer chromatography (TLC) and extracted pigments used for bacterial biofilm inhibition studies. The quantitative distribution of carotenoid substances in the skull and exterior covering body parts of shrimp exhibit low carotenoid intensities (165.1 µg/g and 150.7 µg/g, respectively). Isolated pigments showed a unique single band (orange in color) with retention times ranging from 2.9 to 3.09 min in reversed phase high‐performance liquid chromatography. Functional group arrangement was investigated by Fourier transform infrared spectroscopy and globular nature visualized through scanning electron microscopy. Shrimp carotenoids presented higher microbial inhibitory action against gram‐positive strains than against gram‐negative strains. Biofilm inhibitory concentration was determined for these bacterial strains and biofilm suppression was established and envisaged through in situ microscopic analysis.

中文翻译：

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虾中生物活性色素类胡萝卜素的鉴定，表征及其生物膜抑制作用

从3种对虾（Fenneropenaeus indicus，Metapenaeus ensis和Penaeus semisulcatus）的不同身体成分（头部和甲壳）中分离出生物活性类胡萝卜素。），使用薄层色谱（TLC）和提取的色素进行细菌生物膜抑制研究。虾的颅骨和外壳外部的类胡萝卜素物质的定量分布显示出低的类胡萝卜素强度（分别为165.1 µg / g和150.7 µg / g）。在反相高效液相色谱中，分离出的颜料显示出独特的单一谱带（颜色为橙色），保留时间为2.9至3.09分钟。通过傅立叶变换红外光谱研究官能团的排列，并通过扫描电子显微镜观察球状性质。虾类胡萝卜素对革兰氏阳性菌株的抑制作用要强于对革兰氏阴性菌株的抑制作用。