Anti-inflammatory and antioxidant effects of Apium graveolens L. extracts mitigate against fatal acetaminophen-induced acute liver toxicity.,Journal of Food Biochemistry

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Anti-inflammatory and antioxidant effects of Apium graveolens L. extracts mitigate against fatal acetaminophen-induced acute liver toxicity.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-07-26 , DOI: 10.1111/jfbc.13399
Ayat M Emad ₁ , Sherifa F Ali _{1,

2} , Engy A Abdel-Rahman _{3,

4} , Meselhy R Meselhy ₂ , Mohamed A Farag _{2,

5} , Sameh S Ali _{3,

6} , Essam A Abdel-Sattar ₂

Affiliation

In the present work, antioxidant activity, total phenolics (TP), and total flavonoids (TF) contents of aqueous and methanol extracts of celery were determined, in addition to untargeted metabolites profiling its methanol celery root extract (MCRE) via UPLC‐MS. Although MCRE exhibited the lowest TPC and TFC levels, it presented the most potential hydroxyl radical quenching effect using electron paramagnetic resonance spin trapping technique. Treatment of Acetaminophen‐induced hepatotoxicity (AAH) rats with MCRE lowered serum levels of AST, ALT, ALP, TNF‐α, and IL‐1β significantly. Additionally, MCRE significantly increased total antioxidant capacity (TAC) and glutathione (GSH) levels relative to AAH rats. Strikingly, Kaplan–Meier survival analysis of all groups revealed a 100% prevention of acetaminophen‐induced mortality of rats by MCRE pretreatment (100 mg/kg/day). MCRE prevented AAH‐associated severe weight loss and elicited normal behavior in the rescued rats. Our results suggest that pretreatment with MCRE can mitigate against overdosed acetaminophen‐induced acute liver failure and warrant further investigations on the potential of postinjury intervention.

中文翻译：

芹菜提取物的抗炎和抗氧化作用可减轻对乙酰氨基酚引起的致命急性肝毒性。

在目前的工作中，除了通过 UPLC-MS 分析芹菜甲醇根提取物 (MCRE) 的非目标代谢物外，还测定了芹菜水提取物和甲醇提取物的抗氧化活性、总酚 (TP) 和总黄酮 (TF) 含量。尽管MCRE表现出最低的TPC和TFC水平，但它利用电子顺磁共振自旋捕获技术呈现出最有潜力的羟基自由基猝灭效应。用 MCRE 治疗对乙酰氨基酚诱导的肝毒性 (AAH) 大鼠可显着降低 AST、ALT、ALP、TNF-α 和 IL-1β 的血清水平。此外，相对于 AAH 大鼠，MCRE 显着提高了总抗氧化能力 (TAC) 和谷胱甘肽 (GSH) 水平。引人注目的是，所有组的 Kaplan-Meier 生存分析显示，MCRE 预处理（100 毫克/公斤/天）可 100% 预防对乙酰氨基酚引起的大鼠死亡。 MCRE 防止了 AAH 相关的严重体重减轻，并引发了获救大鼠的正常行为。我们的结果表明，MCRE 预处理可以减轻过量对乙酰氨基酚引起的急性肝衰竭，并值得进一步研究损伤后干预的潜力。

更新日期：2020-07-26

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