This research aims to evaluate the mechanical properties, biocompatibility, and degradation behavior of scaffolds made of pure hydroxyapatite (HA) and HA‐modified by ZnO for bone tissue engineering applications. HA and ZnO were developed using sol‐gel and precipitation methods respectively. The scaffolds properties were characterized using X‐ray diffraction (XRD), Fourier transform spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), atomic absorption (AA), and atomic force microscopy (AFM). The interaction of scaffold with cells was assessed using in vitro cell proliferation and alkaline phosphatase (ALP) assays. The obtained results indicate that the HA/ZnO scaffolds possess higher compressive strength, fracture toughness, and density—but lower hardness—when compared to the pure HA scaffolds. After immersing the scaffold in the SBF solution, more deposited apatite appeared on the HA/ZnO, which results in the rougher surface on this scaffold compared to the pure HA scaffold. Finally, the in vitro biological analysis using human osteoblast cells reveals that scaffolds are biocompatible with adequate ALP activity.

中文翻译：

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纳米羟基磷灰石和纳米羟基磷灰石/氧化锌支架在骨组织工程中的应用

这项研究旨在评估由纯羟基磷灰石（HA）和经ZnO改性的HA制成的支架在骨组织工程应用中的力学性能，生物相容性和降解行为。HA和ZnO分别使用溶胶-凝胶法和沉淀法开发。使用X射线衍射（XRD），傅里叶变换光谱（FTIR），扫描电子显微镜（SEM），能量色散光谱（EDS），透射电子显微镜（TEM），原子吸收（AA）和原子表征了支架的性能力显微镜（AFM）。使用体外细胞增殖和碱性磷酸酶（ALP）分析评估支架与细胞的相互作用。所得结果表明，HA / ZnO支架具有较高的抗压强度，断裂韧性，与纯HA支架相比，密度和密度更低，但硬度更低。将支架浸入SBF溶液后，更多的沉积磷灰石出现在HA / ZnO上，与纯HA支架相比，此支架的表面更粗糙。最后，使用人成骨细胞进行的体外生物学分析表明，支架具有足够的ALP活性，具有生物相容性。