The advent of modern “omics” technologies (genomics, transcriptomics, proteomics, and metabolomics) are attributed to innovative breakthroughs in genome sequencing, bioinformatics, and analytic tools. An organism's biological structure and function is the result of the concerted action of single cells in different tissues. Single cell genomics has emerged as a ground‐breaking technology that has greatly enhanced our understanding of the complexity of gene expression at a microscopic resolution and holds the potential to revolutionize the way we characterize complex cell assemblies and study their spatial organization, dynamics, clonal distribution, pathways, function, and networking. Mammalian systems have benefitted immensely from these approaches to dissect complex systems such as cancer, immunological disorders, epigenetic controls of diseases, and understanding of developmental biology. However, the applications of single‐cell omics in plant research are just starting. The potential to decipher the fundamentals of developmental and functional biology of large and complex plant species at the single‐cell resolution are now becoming important drivers of research. In this review, we present the status, challenges and potential of one important and most commonly used single‐cell omics technique in plants, namely single cell transcriptomics. © 2020 International Society for Advancement of Cytometry

中文翻译：

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单细胞转录组学在了解植物发育和功能生物学方面的现状和潜力。

现代“组学”技术（基因组学、转录组学、蛋白质组学和代谢组学）的出现归功于基因组测序、生物信息学和分析工具方面的创新突破。生物体的生物结构和功能是不同组织中单个细胞协同作用的结果。单细胞基因组学已成为一项突破性技术，它极大地增强了我们对微观分辨率基因表达复杂性的理解，并有可能彻底改变我们表征复杂细胞组装和研究其空间组织、动力学、克隆分布的方式、途径、功能和网络。哺乳动物系统从这些解剖复杂系统的方法中受益匪浅，例如癌症、免疫疾病、疾病的表观遗传控制，以及对发育生物学的理解。然而，单细胞组学在植物研究中的应用才刚刚开始。在单细胞分辨率下破译大型复杂植物物种发育和功能生物学基础的潜力现在正成为研究的重要驱动力。在这篇综述中，我们介绍了植物中一种重要且最常用的单细胞组学技术，即单细胞转录组学的现状、挑战和潜力。© 2020 国际细胞计量学促进会 在单细胞分辨率下破译大型复杂植物物种发育和功能生物学基础的潜力现在正成为研究的重要驱动力。在这篇综述中，我们介绍了植物中一种重要且最常用的单细胞组学技术，即单细胞转录组学的现状、挑战和潜力。© 2020 国际细胞计量学促进会 在单细胞分辨率下破译大型复杂植物物种发育和功能生物学基础的潜力现在正成为研究的重要驱动力。在这篇综述中，我们介绍了植物中一种重要且最常用的单细胞组学技术，即单细胞转录组学的现状、挑战和潜力。© 2020 国际细胞计量学促进会