Abstract

The paper reports the investigation of the population structure of Yersinia pestis strains belonging to the ulegeica subspecies (genetic branch 0.PE5), which is endemic to the natural plague foci in Mongolia and which is characterized by low epidemic potential compared to the strains belonging to the main plague subspecies. Whole genome SNP analysis of 12 Y. pestis subspecies ulegeica strains isolated in Mongolia considering the 1581 identified core SNPs revealed two main genetic clusters in correspondence with the geographic distribution of these strains. The first cluster, named 0.PE5/1, contains the strains isolated in the Umnegov’, Uverkhangai, and Gov’-Altai aimaks located in the south of Mongolia. The second cluster 0.PE5/2 comprises two subclusters, namely, 0.PE5/2-1, containing the strains from the Bayan-Ulgii and Khovd aimaks, and 0.PE5/2-2, represented solely by the strains isolated in the Bayan-Ulgii aimak. To find the possible causes of the selective virulence of subspecies ulegeica strains, the analysis of the nucleotide sequences of 79 pathogenicity factors along with some other genomic regions has been carried out. Specific mutations have been found in the following genes: irp1, included in the high-pathogenicity island of the chromosomal pigmentation region, and yscQ, involved in the regulation of the synthesis of the LcrV protein and outer membrane proteins (Yops) associated with plague pathogen virulence.

中文翻译：

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鼠疫耶尔森菌亚种ulegeica菌株的系统地理分析

摘要

本文报道了鼠疫耶尔森氏菌菌株ulegeica亚种（遗传分支0.PE5）的种群结构调查，该菌株是蒙古的自然鼠疫疫源地，与该菌株相比具有较低的流行潜力。主要的鼠疫亚种。12个鼠疫耶尔森菌亚种ulegeica的全基因组SNP分析考虑到1581个已鉴定的核心SNP，在蒙古分离的菌株显示出两个主要的遗传簇，与这些菌株的地理分布相对应。第一个簇名为0.PE5 / 1，其中包含在蒙古南部的Umnegov'，Uverkhangai和Gov'-Altai aimaks中分离的菌株。第二个簇0.PE5 / 2包含两个子簇，即包含来自Bayan-Ulgii和Khovd aimaks的菌株的0.PE5 / 2-1，以及仅由分离的菌株代表的0.PE5 / 2-2。 Bayan-Ulgii aimak。为了找到可能的ulegeica亚种菌株的选择性毒力的原因，已经对79种致病因子以及其他一些基因组区域的核苷酸序列进行了分析。在以下基因中发现了特定的突变：IRP1，包括在高致病岛的染色体色素沉着区域，并且yscQ，参与LcrV蛋白和鼠疫病原体的毒力相关的外膜蛋白（Yops）的合成中的调节。