Abstract

Small RNAs, especially microRNAs (miRNAs), play an essential role in the responses and adaptations of plants to many stresses, including salt stress. Characterizing miRNAs that correlate with salt stress will improve our understanding of salt tolerance and thus contribute to the sustainable production of maize. In our previous research, small RNA sequencing and degradome sequencing was performed on a maize inbred line (LH196), and many salt stress-responsive miRNAs and their targets were identified. In this study, the identity of target genes of miR164a was confirmed by quantitative expression analysis. Furthermore, the interactions between miR164a and its target genes were examined in a preliminary experiment using overexpression and target mimicry under salt treatment in maize. The results show that Zma-miR164 may respond to salt stress; because the expression of zma-miR164 was decreased with salt stress, leading to a significant increase in the expression of its target genes GRMZM2G114850 (the NAC transcription factor family proteins NAM, ATAF, and CUC) and GRMZM2G008819 (electron carrier). The NAC transcription factors respond to salt stress by regulating the expression pattern of their downstream genes.

中文翻译：

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玉米miR164在盐胁迫响应中的调控机制

摘要

小RNA，尤其是微RNA（miRNA），在植物对多种胁迫（包括盐胁迫）的响应和适应中起着至关重要的作用。表征与盐胁迫相关的miRNA将改善我们对盐耐性的理解，从而有助于玉米的可持续生产。在我们之前的研究中，在玉米自交系（LH196）上进行了小RNA测序和降解组测序，并鉴定了许多盐胁迫反应性miRNA及其靶标。在这项研究中，通过定量表达分析证实了miR164a靶基因的身份。此外，在玉米中，在盐处理下使用过表达和目标拟态进行了初步实验，研究了miR164a及其目标基因之间的相互作用。结果表明，Zma-miR164可能对盐胁迫响应。因为zma-miR164的表达随着盐胁迫而降低，导致其靶基因GRMZM2G114850（NAC转录因子家族蛋白NAM，ATAF和CUC）和GRMZM2G008819（电子载体）的表达显着增加。NAC转录因子通过调节其下游基因的表达模式来响应盐胁迫。