In this study, rumen content was used to obtain three enrichments of anaerobic fungi and methanogens (F + M enrichment), bacteria and methanogens (B + M enrichment), and whole rumen content (WRC enrichment), to evaluate their respective ability to degrade lignocellulose and produce methane. Among the treatments, F + M enrichment elicited the strongest lignocellulose degradation and methane production ability with both rice straw and wheat straw as substrates. Quantitative real-time PCR analysis and diversity analyses of methanogens in the three enrichment treatments demonstrated that F + M had larger number of 16S rRNA gene copies of methanogens and higher relative abundance of Methanobrevibacter, the predominant methanogen found in all enrichments. Caecomyces was the main anaerobic fungal genus for co-culturing to provide substrates for methanogens in this enrichment. Importantly, the F + M enrichment was stable and could be maintained with transfers supplied every 3 days, confirming its potential utility in anaerobic digestion for lignocellulose degradation and methane production.

中文翻译：

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厌氧真菌和产甲烷菌的富集显示出比细菌和产甲烷菌更高的木质纤维素降解和产甲烷能力

本研究利用瘤胃含量分别获得厌氧真菌和产甲烷菌（F+M 富集）、细菌和产甲烷菌（B+M 富集）和全瘤胃内容物（WRC 富集）三种富集情况，以评价它们各自的降解能力。木质纤维素并产生甲烷。在这些处理中，F+M富集以稻草和小麦秸秆为基质引起了最强的木质纤维素降解和产甲烷能力。三种富集处理中产甲烷菌的定量实时 PCR 分析和多样性分析表明，F + M 具有更多数量的产甲烷菌 16S rRNA 基因拷贝和更高的相对丰度 Methanobrevibacter，这是所有富集中发现的主要产甲烷菌。Caecomyces 是共培养的主要厌氧真菌属，为该富集的产甲烷菌提供底物。重要的是，F + M 富集是稳定的，可以通过每 3 天提供的转移来维持，证实了其在厌氧消化中木质纤维素降解和甲烷生产的潜在效用。