Phomopsis asparagi has been reported as a serious disease, causing stem blight on asparagus cultivars (Asparagus officinalis), and this fungus was also recorded as a common spot disease on wild asparagus (A. kiusianus). In this study, isolates of P. asparagi obtained from A. officinalis and A. kiusianus in different locations of the Kyushu island were successfully assessed the genetic variation through mycelial compatibility grouping (MCG) and random amplified polymorphic DNA (RAPD). Thirty fungal isolates were identified into six MCGs; of these, fifteen isolates were representatively chosen for the RAPD analysis, pathogenicity and extracellular enzyme test. Eight RAPD primers, B62, B66, B68, B71, C83, D22, D32 and E10, generated 100 different patterns from the isolates with 91% of the polymorphic bands. Based on RAPD analysis, P. asparagi isolates were divided into 3 distinct genetic clusters, namely RAPD-I, RAPD-II and RAPD-III at a similarity of 73%. The result indicated that there was a great genetic variation within the isolates from A. kiusianus, presenting in the three clusters. While the isolates from A. officinalis showed high genetic homology and they belonged to the RAPD-II cluster. There is no conflict between genetic clusters distinguished by the RAPD markers and those of MCG. On the other hand, the pathogenicity test revealed that the isolates of RAPD-II and RAPD-III were highly virulent pathogens. In contrast, the isolates of RAPD-I were weak virulent pathogens when inoculated on A. officinalis. Screening of extracellular enzyme activities concluded that all isolates were positive for laccase, lipase and protease activity.

芦笋茎枯已被报告为一种严重的疾病，造成对芦笋品种茎枯病（芦笋），而这种真菌也被记录为野生芦笋（一种常见的斑病A. kiusianus）。在这项研究中，从A. officinalis和A. kiusianus中获得的天冬孢菌的分离株通过菌丝相容性分组法（MCG）和随机扩增多态性DNA（RAPD）成功地评估了九州岛不同地区的遗传变异。将30个真菌分离株鉴定为6个MCG；其中，有代表性的15种分离株用于RAPD分析，致病性和细胞外酶测试。八种RAPD引物B62，B66，B68，B71，C83，D22，D32和E10从具有91％多态性条带的分离物中产生了100种不同的模式。根据RAPD分析，将天冬疟原虫分离株分为3个不同的遗传簇，即RAPD-I，RAPD-II和RAPD-III，相似性为73％。结果表明，A。kiusianus分离物中存在很大的遗传变异。，呈现在三个集群中。虽然来自A. officinalis的分离株显示出高度的遗传同源性，但它们属于RAPD-II簇。通过RAPD标记区分的遗传簇与MCG的遗传簇之间没有冲突。另一方面，致病性测试表明，RAPD-II和RAPD-III的分离株是高毒力病原体。相反，当接种到A.officinalis上时，RAPD-1的分离物是弱毒性的病原体。细胞外酶活性的筛选得出结论，所有分离株的漆酶，脂肪酶和蛋白酶活性均为阳性。