Five previously isolated bacterial strains were cultured on xylan agar medium (XAM) to screen their xylanase production potential. Two best strains were obtained on the basis of clear zones on XAM. The strain, Bacillus licheniformis, produced higher xylanase (3.24 U/ml) was selected for further studies based on quantitative xylanase assay. Both cultural and medium parameters were optimized to yield highest xylanase production. Best cultural conditions found were 5 pH, 1% inoculum size, 37 °C and 24 h of fermentation period. To optimize the xylanase production, medium ingredients viz, banana peels, yeast extract, KH₂PO₄, K₂HPO₄, sodium nitrate, MgSO₄, peptone, NaCl and ammonium sulfate were screened through 12 runs Plackett–Burman design of response surface methodology. From 12 experimental runs, 4 significantly affecting medium ingredients (banana peels, sodium nitrate, peptone, MgSO₄) were selected and further optimized through central composite design. The banana peels (1.5%), sodium nitrate (0.3875%), peptone (0.8%) and MgSO₄ (0.775%) had yielded significantly highest xylanase production. Partial characterization of crude xylanase revealed optimum 8 pH and 55 °C temperature.

中文翻译：

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香蕉皮埋入式发酵中地衣芽孢杆菌木聚糖酶的统计优化

在木聚糖琼脂培养基（XAM）上培养了五个先前分离的细菌菌株，以筛选其木聚糖酶生产潜力。基于XAM上的透明区域，获得了两个最佳菌株。基于定量的木聚糖酶测定法，选择产生更高木聚糖酶（3.24U / ml）的地衣芽孢杆菌（Bacillus licheniformis）菌株用于进一步研究。优化培养基和培养基参数以产生最高的木聚糖酶产量。发现的最佳培养条件为5 pH，1％接种量，37°C和发酵期24 h。为了优化木聚糖酶的生产，应使用中等成分，即香蕉皮，酵母提取物，KH ₂ PO ₄，K ₂ HPO ₄，硝酸钠，MgSO ₄通过12次Plackett-Burman设计的响应面方法筛选了蛋白,、 NaCl和硫酸铵。从12个实验运行中，选择了4种影响显着的中等成分（香蕉皮，硝酸钠，蛋白ept，MgSO ₄），并通过中央复合设计进一步优化。香蕉皮（1.5％），硝酸钠（0.3875％），蛋白ept（0.8％）和MgSO ₄（0.775％）产生了最高的木聚糖酶产量。粗木聚糖酶的部分表征显示最佳的8 pH和55°C温度。