Guangdong bream (Megalobrama terminalis) is an economically important species. However, wild Guangdong bream resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial that the current resources of Guangdong bream are protected. In this study, 30 novel single nucleotide polymorphism (SNP) markers were developed based on restriction site-associated DNA sequencing (RAD-seq). The results showed that the minor allele frequency varied from 0.021 to 0.489 and that the observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.043 to 0.638 and 0.042 to 0.506, respectively. The PIC ranged from 0.041 to 0.375. Seven loci showed significant deviations from the HWE after Bonferroni correction (p < 0.05), but no significant linkage disequilibrium was found. These SNP markers will serve as a useful tool for genetic studies and population evaluation aimed at the conservation of Guangdong bream.

广东鲷（Megalobrama terminalis）是经济上重要的物种。但是，近年来，由于人为干扰和栖息地破坏，野生广东鲷资源急剧减少。因此，保护​​广东鲷的现有资源至关重要。在这项研究中，基于限制性位点相关的DNA测序（RAD-seq），开发了30种新颖的单核苷酸多态性（SNP）标记。结果表明，次要等位基因频率在0.021至0.489之间变化，观察到的杂合度（Ho）和预期杂合度（He）分别在0.043至0.638和0.042至0.506之间。PIC的范围从0.041到0.375。Bonferroni校正后，有七个基因座显示出与HWE的显着偏离（p <0.05），但未发现显着的连锁不平衡。