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Single nucleotide polymorphism markers for genotyping hawksbill turtles ( Eretmochelys imbricata )
Conservation Genetics Resources ( IF 0.9 ) Pub Date : 2019-09-05 , DOI: 10.1007/s12686-019-01112-z
Shreya M. Banerjee , Lisa M. Komoroske , Amy Frey , Brittany Hancock-Hanser , Phillip A. Morin , Frederick I. Archer , Suzanne Roden , Alexander Gaos , Michael J. Liles , Peter H. Dutton

Assessing population connectivity and identifying conservation units are critical to formulating effective management plans for marine turtles. We combined a multiplexed targeted enrichment capture technique and high-throughput sequencing to discover single nucleotide polymorphisms (SNPs) and to genotype hawksbill turtles (Eretmochelys imbricata) to examine population structure. Our approach discovered 455 potential informative SNPs for hawksbill turtles. We optimized 25 of the 455 SNPs discovered and used them to identify preliminary evidence of hawksbill population structure, suggesting that these markers will be informative in discerning conservation units when applied to larger sample sizes in future studies.



中文翻译:

基因型的单核苷酸多态性标记(Eretmochelys imbricata)

评估种群连通性并确定保护单位对于制定有效的海龟管理计划至关重要。我们结合了多重靶向富集捕获技术和高通量测序技术,以发现单核苷酸多态性(SNP)和基因型(Eretmochelys imbricata)来研究种群结构。我们的方法为发现了455个潜在的信息丰富的SNP。我们对发现的455个SNP中的25个进行了优化,并用它们来确定种群的初步证据,这表明这些标记物在将来的研究中应用于较大的样本量时,将有助于识别养护单位。

更新日期:2019-09-05
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