Yellowtail kingfish, Seriola lalandi, is a globally distributing pelagic marine fish species. In recent years, the wild population has suffered drastic decline due to overfishing and environmental deterioration. Thus, the sustainable utilization and conservation management for this species should be implemented urgently. In the present study, we developed SNP markers using 2b-RAD sequencing method from 33 individuals of yellowtail kingfish. Wherein, 100 high-quality SNP markers were obtained based on high throughput sequencing and stringent SNP filter process. The minor allele frequency of these SNPs ranged from 0.0126 to 0.4957. The observed heterozygosity and expected heterozygosity ranged from 0 to 0.9914 and 0.0249 to 0.5000, respectively. The inbreeding coefficient varied from − 0.9828 to 0.7882. Among these SNPs, 10 loci deviated significantly from Hardy–Weinberg equilibrium after Bonferroni correction. These novel SNP markers will serve as valuable tools for population genetics evaluation and natural resources conservation of yellowtail kingfish.

黄尾翠鸟，Seriola lalandi是全球分布的中上层海洋鱼类物种。近年来，由于过度捕捞和环境恶化，野生种群急剧减少。因此，应紧急实施该物种的可持续利用和保护管理。在本研究中，我们使用2b-RAD测序方法从33尾yellow鱼中开发了SNP标记。其中，基于高通量测序和严格的SNP过滤过程，获得了100个高质量的SNP标记。这些SNP的次要等位基因频率为0.0126至0.4957。观察到的杂合度和预期杂合度分别为0至0.9914和0.0249至0.5000。近交系数从− 0.9828到0.7882。在这些SNP中，Bonferroni校正后，有10个基因座明显偏离Hardy-Weinberg平衡。这些新颖的SNP标记将为黄尾king的种群遗传学评估和自然资源保护提供有价值的工具。