Cerebral microdialysis (CMD) is a minimally invasive technique for sampling the interstitial fluid in human brain tissue. CMD allows monitoring the metabolic state of tissue, as well as sampling macromolecules such as proteins and peptides. Recovery of proteins or peptides can be hampered by their adsorption to the CMD membrane as has been previously shown in-vitro, however, protein adsorption to CMD membranes has not been characterized following implantation in human brain tissue. In this paper, we describe the pattern of proteins adsorbed to CMD membranes compared to that of the microdialysate and of cerebrospinal fluid (CSF). We retrieved CMD membranes from three surgically treated intracerebral hemorrhage (ICH) patients, and analyzed protein adsorption to the membranes using two-dimensional gel electrophoresis (2-DE) in combination with nano-liquid mass spectrometry. We compared the proteome profile of three compartments; the CMD membrane, the microdialysate and ventricular CSF collected at time of CMD removal. We found unique protein patterns in the molecular weight range of 10–35 kDa for each of the three compartments. This study highlights the importance of analyzing the membranes in addition to the microdialysate when using CMD to sample proteins for biomarker investigation.

中文翻译：

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蛋白质组学研究在手术治疗的脑出血患者中对脑微透析膜的蛋白质吸附 - 一项初步研究。

脑微透析 (CMD) 是一种微创技术，用于对人脑组织中的间质液进行采样。CMD 允许监测组织的代谢状态，以及对蛋白质和肽等大分子进行采样。蛋白质或肽的回收可能会因它们吸附到 CMD 膜而受到阻碍，如先前在体外显示的那样，然而，在植入人脑组织后尚未表征蛋白质或肽对 CMD 膜的吸附。在本文中，我们描述了与微透析液和脑脊液 (CSF) 相比，吸附到 CMD 膜上的蛋白质模式。我们从三名经过手术治疗的脑出血 (ICH) 患者身上取出 CMD 膜，并使用二维凝胶电泳（2-DE）结合纳米液体质谱分析蛋白质对膜的吸附。我们比较了三个隔间的蛋白质组谱；去除 CMD 时收集的 CMD 膜、微透析液和心室脑脊液。我们发现三个隔间中每一个的独特蛋白质模式在 10-35 kDa 的分子量范围内。本研究强调了在使用 CMD 对蛋白质进行采样以进行生物标志物研究时，除微透析液外还分析膜的重要性。