With the high demand for diesel across the world, environmental decontamination from its improper usage, storage and accidental spills becomes necessary. One highly environmentally friendly and cost-effective decontamination method is to utilize diesel-degrading microbes as a means for bioremediation. Here, we present a newly isolated and identified strain of Acinetobacter calcoaceticus (‘CA16’) as a candidate for the bioremediation of diesel-contaminated areas. Acinetobacter calcoaceticus CA16 was able to survive and grow in minimal medium with diesel as the only source of carbon. We determined through metabolomics that A. calcoaceticus CA16 appears to be efficient at diesel degradation. Specifically, CA16 is able to degrade 82 to 92% of aliphatic alkane hydrocarbons (CnHn + 2; where n = 12–18) in 28 days. Several diesel-degrading genes (such as alkM and xcpR) that are present in other microbes were also found to be activated in CA16. The results presented here suggest that Acinetobacter strain CA16 has good potential in the bioremediation of diesel-polluted environments.

中文翻译：

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从加拿大土壤中分离出的柴油降解细菌，不动杆菌钙钙乙酸CA16的特征和基因组分析。

随着全世界对柴油的高需求，由于不当使用，储存和意外泄漏而造成的环境污染变得必要。一种高度环保且具有成本效益的净化方法是利用柴油降解微生物作为生物修复手段。在这里，我们提出了一种新分离和鉴定出的不动杆菌钙乙酸不动杆菌（'CA16'）菌株，作为对柴油污染地区进行生物修复的候选对象。醋酸钙不动杆菌CA16能够在以柴油为唯一碳源的基本培养基中存活并生长。我们通过代谢组学确定了A. calcoaceticus CA16似乎对柴油降解有效。具体来说，CA16能够在28天内降解82％至92％的脂肪族烷烃（CnHn + 2；其中n = 12-18）。其他微生物中存在的几种柴油降解基因（例如alkM和xcpR）也被发现在CA16中被激活。此处显示的结果表明，不动杆菌菌株CA16在柴油污染环境的生物修复中具有良好的潜力。